How can you use size exclusion chromatography to determine the size of an unknown protein in a mixture?

• A. By determining its solubility and comparing it to a standard curve of proteins with known solubility.
• B. By measuring its absorbance and comparing it to a standard curve of proteins with known charges.
• C. By analyzing its color and comparing it to a standard curve of proteins with known colors.
• D. By comparing its elution time to a standard curve of proteins with known sizes.

Answer: (D)