A biochemist is analyzing a protein sample at two different wavelengths, 260 nm and 280 nm. The absorbance at 280 nm is significantly higher. What can be inferred about the molar extinction coefficient at these wavelengths?

• A. The molar extinction coefficient is the same at both wavelengths.
• B. The molar extinction coefficient does not affect absorbance.
• C. The molar extinction coefficient is higher at 260 nm.
• D. The molar extinction coefficient is higher at 280 nm.

Answer: (D)