Why does the slope of the Lineweaver-Burk plot remain unchanged in the presence of uncompetitive inhibitors?

• A. Because the inhibitor only affects the free enzyme.
• B. Because the substrate concentration is not affected.
• C. Because uncompetitive inhibitors do not affect enzyme kinetics.
• D. Because both Km and Vmax are proportionally decreased, keeping the Km/Vmax ratio constant.

Answer: (D)