Question 1

Which of the following best describes the stationary phase in column chromatography?

Accepted Answer

A solid material that is immobile and allows for separation based on differential affinities.

Question 2

In column chromatography, what is the primary reason different components of a mixture separate as they move through the column?

Accepted Answer

They have different affinities for the stationary phase.

Question 3

Which of the following equations best represents the partition coefficient ($$K) in $$chromatography?

Accepted Answer

$$K = \frac{[solute]_{stationary}}{[solute]_{mobile}}$$

Question 4

A researcher wants to purify a protein mixture using column chromatography. Which property of the proteins will most directly affect their order of elution from the column?

Accepted Answer

Their relative affinity for the stationary phase.

Question 5

Which of the following statements about the mobile phase in column chromatography is correct?

Accepted Answer

It is the solvent that moves through the column, carrying the sample with it.

Question 6

A mixture contains two compounds: A (high affinity for stationary phase) and B (low affinity for stationary phase). Which will elute first from the column?

Accepted Answer

Compound B

Question 7

Which of the following techniques directly allows for effective separation/purification of a target protein?

Accepted Answer

Chromatography

Question 8

Why is chromatography considered an expensive technique for large samples?

Accepted Answer

It requires more materials and time as sample size increases.

Question 9

Which of the following best explains why chromatography is often used as a final purification step?

Accepted Answer

It provides the most effective separation when the sample size is small.

Question 10

A scientist observes that a compound remains at the top of the column during chromatography. What is the most likely explanation?

Accepted Answer

The compound has a very high affinity for the stationary phase.

Question 11

Which of the following would NOT be a reason for poor separation in a column chromatography experiment?

Accepted Answer

Using a stationary phase with high specificity for one component.

Question 12

If a researcher wants to separate two proteins with very similar sizes but different charges, which type of chromatography would be most effective?

Accepted Answer

Ion-exchange chromatography

Question 13

Which of the following best describes the role of the eluent in column chromatography?

Accepted Answer

It is the mobile phase that helps carry the sample through the column.

Question 14

A student sets up a column chromatography experiment but observes no separation of the sample. Which of the following is the most likely cause?

Accepted Answer

The stationary phase does not interact differently with the sample components.

Question 15

Which of the following is a key advantage of using chromatography for protein purification?

Accepted Answer

It allows for separation based on specific molecular properties such as size, charge, or affinity.

Column Chromatography: Principles and Applications

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