$\mathrm{Glucose + ATP \rightarrow Glucose\ 6\text{-}phosphate + ADP + H^+}$

Question 1

Which enzyme catalyzes the phosphorylation of glucose to glucose 6-phosphate in the first step of glycolysis?

Accepted Answer

Hexokinase

Question 2

What is the net chemical equation for the conversion of glucose to glucose 6-phosphate in glycolysis?

Accepted Answer

$\mathrm{Glucose + ATP ightarrow Glucose\ 6	ext{-}phosphate + ADP + H^+}$

Question 3

Which of the following best describes the role of phosphoglucose isomerase in glycolysis?

Accepted Answer

It converts glucose 6-phosphate into fructose 6-phosphate.

Question 4

During the third step of glycolysis, which molecule acts as the phosphate donor?

Accepted Answer

ATP

Question 5

What is the product of the reaction catalyzed by phosphofructokinase-1?

Accepted Answer

Fructose 1,6-bisphosphate

Question 6

Which glycolytic enzyme catalyzes the cleavage of a six-carbon sugar into two three-carbon sugars?

Accepted Answer

Aldolase

Question 7

Which of the following is a direct product of the aldolase reaction in glycolysis?

Accepted Answer

Glyceraldehyde 3-phosphate

Question 8

If a mutation in the phosphofructokinase-1 gene reduces its activity, what is the most likely immediate effect on glycolysis?

Accepted Answer

Accumulation of fructose 6-phosphate

Question 9

Which step in glycolysis is considered the committed step, and why?

Accepted Answer

The conversion of fructose 6-phosphate to fructose 1,6-bisphosphate, because it is highly regulated and irreversible.

Question 10

Which of the following best explains why ATP is required in the early steps of glycolysis?

Accepted Answer

ATP provides phosphate groups to activate glucose and fructose 6-phosphate.

Question 11

Which of the following reactions in glycolysis is an example of an isomerization?

Accepted Answer

Glucose 6-phosphate $\rightarrow$ Fructose 6-phosphate

Question 12

A patient has a deficiency in aldolase. Which glycolytic intermediate would you expect to accumulate?

Accepted Answer

Fructose 1,6-bisphosphate

Question 13

Which of the following best describes the energy investment phase of glycolysis?

Accepted Answer

It consumes two ATP molecules per glucose molecule.

Question 14

Which enzyme catalyzes the reaction: $\mathrm{Fructose\ 6	ext{-}phosphate + ATP ightarrow Fructose\ 1,6	ext{-}bisphosphate + ADP + H^+}$?

Accepted Answer

Phosphofructokinase-1

Question 15

Which of the following is NOT a product of the aldolase reaction in glycolysis?

Accepted Answer

Fructose 1,6-bisphosphate

Glycolysis: Key Steps and Enzymatic Reactions

Study Guide - Practice Questions