Question 1

Which physical or chemical property is NOT commonly used for protein separation in chromatography?

Accepted Answer

Molecular polarity

Question 2

In ion exchange chromatography, which type of exchanger would you use to bind negatively charged proteins?

Accepted Answer

Anion exchanger

Question 3

A protein mixture is loaded onto a column containing DEAE (diethylaminoethyl) groups. Which proteins will bind to the column?

Accepted Answer

Proteins with a net negative charge

Question 4

Which equation best describes the elution of proteins from an ion exchange column as salt concentration increases?

Accepted Answer

$$[Protein]_{eluted} \propto [Salt]_{added}$$

Question 5

During column chromatography, what is the primary function of the fraction collector?

Accepted Answer

To collect different protein fractions as they elute from the column

Question 6

If a protein has a high affinity for a ligand attached to the column matrix, what will happen during chromatography?

Accepted Answer

It will bind to the matrix and elute last

Question 7

Which of the following is TRUE regarding cation exchange chromatography?

Accepted Answer

The matrix contains negatively charged groups

Question 8

A protein with a pI of 5.0 is placed in a buffer at pH 7.0 and loaded onto a cation exchange column. What will happen?

Accepted Answer

It will not bind because it is negatively charged

Question 9

Which of the following is a correct description of the principle behind ion exchange chromatography?

Accepted Answer

Proteins are separated based on their net charge at a given pH

Question 10

A mixture of three proteins (A, B, and C) is separated by column chromatography. Protein A elutes first, followed by B and then C. What property most likely explains this order?

Accepted Answer

Protein A has the weakest interaction with the column matrix

Question 11

Which buffer condition would you use to elute a protein strongly bound to an ion exchange column?

Accepted Answer

Increase the salt concentration

Question 12

Which of the following is NOT a reason for using chromatography in protein purification?

Accepted Answer

To increase the solubility of proteins

Question 13

In a real-world scenario, a researcher wants to purify a protein that binds DNA. Which chromatography method would be most effective?

Accepted Answer

Affinity chromatography using immobilized DNA

Question 14

Which of the following statements about fraction collectors is FALSE?

Accepted Answer

They separate proteins based on their charge

Question 15

A protein sample is loaded onto an anion exchange column at pH 8.0. The protein has a pI of 6.0. What is the charge of the protein under these conditions?

Accepted Answer

Negative

Question 16

Which of the following is the correct chemical group for a cation exchanger used in ion exchange chromatography?

Accepted Answer

Matrix-CH$$_2-$$COO$$^-$$

Question 17

Which property would you exploit to separate proteins using hydrophobic interaction chromatography?

Accepted Answer

Hydrophobicity

Question 18

A protein is eluted from a column and detected by a UV detector. Which equation relates the absorbance to the protein concentration?

Accepted Answer

$$A = \epsilon c l$$

Question 19

Which of the following is a multi-step strategy for purifying a protein from a complex mixture?

Accepted Answer

Combine ion exchange chromatography followed by affinity chromatography

Chapter 3 part 2

Study Guide - Practice Questions

Study Guide - Flashcards