Question 1

Which amino acid is most likely to disrupt an alpha helix due to its rigid structure?

Accepted Answer

Proline

Question 2

What is the primary stabilizing force in a protein’s native conformation?

Accepted Answer

Hydrophobic effect

Question 3

Which disease is caused by a lack of vitamin C, affecting collagen synthesis?

Accepted Answer

Scurvy

Question 4

Given the Hill equation $$\log\left(\frac{Y}{1-Y}\right) = n \log [L] - \log K_d$$, what does a Hill coefficient $$n \u003e 1$$ indicate?

Accepted Answer

Positive cooperativity

Question 5

Which protein is monomeric, non-cooperative, and facilitates O$$_2$$ storage in muscle?

Accepted Answer

Myoglobin

Question 6

What is the effect of BPG (2,3-bisphosphoglycerate) on hemoglobin’s oxygen affinity?

Accepted Answer

Decreases affinity

Question 7

Which class of enzyme catalyzes the transfer of a functional group from one molecule to another?

Accepted Answer

Transferase

Question 8

If the equilibrium constant $$K_{eq}$$ for a reaction is greater than 1, what can be said about the standard free energy change $$\Delta G^\circ$$?

Accepted Answer

$$\Delta G^\circ is $$negative

Question 9

Which of the following statements about the Anfinsen experiment is correct?

Accepted Answer

Amino acid sequence contains all information needed for folding

Question 10

Which amino acid is most likely to be found in the interior of a globular protein due to its hydrophobic nature?

Accepted Answer

Valine

Question 11

What is the main function of chaperone proteins in cells?

Accepted Answer

Facilitate correct folding of other proteins

Question 12

Which of the following best describes the tertiary structure of a protein?

Accepted Answer

Three-dimensional arrangement of all atoms in a single polypeptide chain

Question 13

Which of the following is true about the effect of a catalyst on a chemical reaction?

Accepted Answer

Catalyst lowers the activation energy $$E_a$$

Question 14

Which of the following is a consequence of sickle cell anemia at the molecular level?

Accepted Answer

Substitution of Val for Glu in hemoglobin

Question 15

Which of the following is the most flexible amino acid, often found in turns of protein structures?

Accepted Answer

Glycine

Question 16

What is the role of the iron atom in the heme group of hemoglobin?

Accepted Answer

Binds oxygen reversibly

Question 17

Which of the following best explains why fetal hemoglobin has a higher affinity for oxygen than adult hemoglobin?

Accepted Answer

Fetal hemoglobin binds BPG less strongly

Question 18

Which of the following is NOT a type of interaction stabilizing protein tertiary structure?

Accepted Answer

Peptide bonds

Question 19

Which statement about the quaternary structure of proteins is correct?

Accepted Answer

It involves the arrangement of multiple polypeptide chains

Question 20

If the rate constant $$k$$ for a reaction increases, which of the following is most likely to occur?

Accepted Answer

The activation energy $$E_a$$ has decreased

Protein Structure, Function, and Enzyme Kinetics: Biochemistry Study Notes

Study Guide - Practice Questions