Question 1

Which of the following compounds is an intermediate of β-oxidation?

Accepted Answer

CH\_3CH\_2CH\_2CO-S-CoA

Question 2

During β-oxidation, why does the reduction of a C=C double bond in certain fatty acids require NADPH?

Accepted Answer

Because the double bond is in a specific cis configuration that standard β-oxidation enzymes cannot process.

Question 3

Consider the β-oxidation of the following fatty acid: $	ext{hexadecanoic acid (16:0)}$. How many rounds of β-oxidation are necessary to convert it to acetyl-CoA?

Accepted Answer

7 rounds

Question 4

What is the total output of reduced electron carriers and ATP from the complete β-oxidation of palmitoyl-CoA ($	ext{C}_{16}$ fatty acid)?

Accepted Answer

7 NADH, 7 FADH\_2, 8 acetyl-CoA, 14 NADH, 14 FADH\_2, 106 ATP

Question 5

Which step in β-oxidation produces FADH\_2?

Accepted Answer

Oxidation of the fatty acyl-CoA to trans-Δ\^2-enoyl-CoA

Question 6

A patient has a deficiency in the enzyme enoyl-CoA isomerase. Which process will be directly affected?

Accepted Answer

Conversion of cis-Δ\^3-enoyl-CoA to trans-Δ\^2-enoyl-CoA during β-oxidation

Question 7

Which of the following best explains why unsaturated fatty acids require additional enzymes for β-oxidation?

Accepted Answer

Because the double bonds are in positions not recognized by standard β-oxidation enzymes

Question 8

During β-oxidation, which molecule is released after each round?

Accepted Answer

Acetyl-CoA

Question 9

If a fatty acid contains an odd number of carbons, what is the final product after β-oxidation?

Accepted Answer

Propionyl-CoA

Question 10

Which cofactor is required for the reduction of a cis double bond in polyunsaturated fatty acids during β-oxidation?

Accepted Answer

NADPH

Question 11

A mutation in the gene encoding acyl-CoA dehydrogenase would most directly affect which step of β-oxidation?

Accepted Answer

Formation of trans-Δ\^2-enoyl-CoA

Question 12

Which of the following is NOT a product of one round of β-oxidation?

Accepted Answer

Malonyl-CoA

Question 13

What is the role of thiolase in β-oxidation?

Accepted Answer

Cleaves the β-ketoacyl-CoA to release acetyl-CoA

Question 14

Which enzyme is responsible for the hydration step in β-oxidation?

Accepted Answer

Enoyl-CoA hydratase

Question 15

How many ATP molecules are generated from the complete oxidation of one molecule of palmitate ($	ext{C}_{16}$ fatty acid)?

Accepted Answer

106 ATP

Question 16

Which of the following statements about β-oxidation is TRUE?

Accepted Answer

It produces acetyl-CoA, NADH, and FADH\_2.

Study Notes on β-Oxidation of Fatty Acids

Study Guide - Practice Questions

Study Guide - Flashcards