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General Biology: Cell Biology and Membranes

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  • Cell Theory

    All living things are composed of cells and all cells come from other cells.

  • Magnification vs Resolution

    Magnification increases image size; Resolution measures image clarity and the ability to distinguish two close objects.

  • Light Microscope (LM)

    Uses visible light and glass lenses to magnify specimens; resolution limit about 0.2μm; max useful magnification ~1000X.

  • Electron Microscopes (EM)

    Use electron beams for higher resolution; include Scanning EM (SEM) for 3D surface images and Transmission EM (TEM) for internal structures.

  • Prokaryotic Cells

    Small, simple cells without membrane-bound organelles; contain a nucleoid region with DNA; examples include bacteria and archaea.

  • Structures Common to All Cells

    Plasma membrane, ribosomes, cytosol, DNA, and cytoplasm.

  • Cell Wall in Prokaryotes

    Rigid, chemically complex shell outside the plasma membrane; protects and maintains cell shape.

  • Capsule in Prokaryotes

    Sticky outer coat that helps cells adhere to surfaces or other cells; not present in all prokaryotes.

  • Flagella

    Long projections that propel cells through their environment; found in some prokaryotes and animal sperm cells.

  • Eukaryotic Cell Organelles

    Membrane-bound structures including nucleus, ER, Golgi apparatus, lysosomes, mitochondria, chloroplasts, cytoskeleton, and more.

  • Nucleus Function

    Contains genetic material (DNA) and controls cell activities by directing protein synthesis.

  • Ribosomes

    Non-membranous organelles that synthesize proteins; free ribosomes make cytosolic proteins, bound ribosomes make proteins for export or membranes.

  • Endoplasmic Reticulum (ER)

    Network of membranes; Rough ER has ribosomes and synthesizes proteins; Smooth ER synthesizes lipids, stores calcium, and detoxifies.

  • Golgi Apparatus

    Processes, modifies, and ships molecules produced by the ER; adds molecular tags and directs products to destinations.

  • Lysosomes

    Membrane sacs with digestive enzymes; digest food, recycle damaged components, and destroy pathogens.

  • Mitochondria

    Site of cellular respiration; double membrane with folded inner membrane (cristae) to increase surface area for ATP production.

  • Chloroplasts

    Organelles for photosynthesis in plants; contain thylakoids with chlorophyll and stroma with DNA and enzymes.

  • Endosymbiont Theory

    Mitochondria and chloroplasts originated as prokaryotes engulfed by early eukaryotic cells, forming a symbiotic relationship.

  • Cytoskeleton

    Network of protein fibers (microtubules, microfilaments, intermediate filaments) that support cell shape, enable movement, and organize organelles.

  • Plasma Membrane Structure

    Fluid mosaic model: phospholipid bilayer with embedded proteins; selectively permeable to regulate material flow.

  • Diffusion and Passive Transport

    Movement of molecules down their concentration gradient without energy; includes simple diffusion and facilitated diffusion via transport proteins.

  • Osmosis and Tonicity

    Osmosis is water diffusion across membranes; tonicity describes solute concentration effects: isotonic (equal), hypotonic (lower outside), hypertonic (higher outside).

  • Active Transport

    Energy-requiring process moving solutes against concentration gradient using transport proteins and ATP (e.g., sodium-potassium pump).

  • Endocytosis and Exocytosis

    Processes for bulk transport: endocytosis brings materials into the cell; exocytosis expels materials via vesicles.

  • Enzymes

    Biological catalysts that lower activation energy to speed reactions; highly specific to substrates and have optimal conditions for activity.

  • ATP Role in Cells

    Primary energy carrier; hydrolysis releases energy for cellular work; ATP is renewable by phosphorylation of ADP.

  • Enzyme Inhibition Types

    Competitive inhibitors bind active site; non-competitive inhibitors bind elsewhere causing shape change; feedback inhibition regulates pathways.