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General Biology Exam 5 Review Flashcards

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  • Bacteriophage

    A virus that infects bacteria, used in molecular biology to study gene transfer.

  • Hershey-Chase experiment

    Demonstrated that DNA is the genetic material by using bacteriophages labeled with radioactive isotopes.

  • Nucleotide

    The basic building block of nucleic acids, consisting of a sugar, phosphate group, and nitrogenous base.

  • Polynucleotide

    A polymer made of many nucleotides linked by phosphodiester bonds forming DNA or RNA strands.

  • DNA

    Deoxyribonucleic acid, the molecule that stores genetic information in cells.

  • Gene

    A segment of DNA that codes for a functional product, usually a protein.

  • Chromosome

    A structure of DNA and proteins that contains genetic information; visible during cell division.

  • Chromatin

    The complex of DNA and proteins in the nucleus, which condenses to form chromosomes.

  • Semiconservative model

    DNA replication model where each new DNA molecule contains one original and one new strand.

  • DNA polymerase

    An enzyme that synthesizes new DNA strands by adding nucleotides complementary to the template strand.

  • DNA ligase

    An enzyme that joins Okazaki fragments on the lagging strand during DNA replication.

  • Transcription

    The process of copying a DNA sequence into an RNA molecule.

  • RNA polymerase

    The enzyme that synthesizes RNA from a DNA template during transcription.

  • Translation

    The process of synthesizing a protein from an mRNA template at the ribosome.

  • Ribosome

    A cellular structure where proteins are synthesized by translating mRNA.

  • Codon

    A sequence of three nucleotides in mRNA that specifies an amino acid or stop signal during translation.

  • Gene promoter

    A DNA sequence where RNA polymerase binds to initiate transcription.

  • mRNA

    Messenger RNA that carries genetic information from DNA to the ribosome for protein synthesis.

  • rRNA

    Ribosomal RNA, a structural and catalytic component of ribosomes.

  • tRNA

    Transfer RNA that brings amino acids to the ribosome during protein synthesis.

  • RNA splicing

    The process of removing introns and joining exons in a pre-mRNA transcript.

  • Intron

    Non-coding sequences in a gene that are removed during RNA splicing.

  • Exon

    Coding sequences in a gene that remain in mRNA after splicing and code for protein.

  • Mutagen

    An agent that causes mutations in DNA, such as chemicals or radiation.

  • Gene regulation

    Control of gene expression to increase or decrease the production of gene products.

  • Operon

    A cluster of genes under control of a single promoter and operator, common in prokaryotes.

  • Gene repressor

    A protein that binds to the operator to block transcription of an operon.

  • Gene operator

    A DNA segment where a repressor binds to regulate gene transcription.

  • Cell differentiation

    The process by which cells become specialized in structure and function.

  • Histone

    Proteins around which DNA winds to form nucleosomes, aiding in chromatin structure.

  • Nucleosome

    The basic unit of DNA packaging, consisting of DNA wrapped around histone proteins.

  • X inactivation

    The process by which one X chromosome in female mammals is silenced to balance gene dosage.

  • Epigenetic modification

    Heritable changes in gene expression without altering the DNA sequence, such as DNA methylation.

  • Transcription factor

    A protein that binds to specific DNA sequences to regulate transcription.

  • Alternative splicing

    A process allowing a single gene to code for multiple proteins by splicing mRNA differently.

  • Micro RNAs

    Small RNA molecules that regulate gene expression by degrading mRNA or blocking translation.

  • Homeotic gene

    Genes that control the body plan and the formation of structures during development.

  • Nucleic acid hybridization

    A technique to detect specific DNA or RNA sequences by base pairing with a labeled probe.

  • DNA microarray

    A tool to measure the expression of many genes simultaneously using hybridization on a chip.

  • Signal transduction pathway

    A series of molecular events by which a cell responds to external signals.

  • Proto-oncogene

    A normal gene that can become an oncogene due to mutations or increased expression.

  • Oncogene

    A mutated or overexpressed gene that can cause cancer by promoting uncontrolled cell growth.

  • Tumor suppressor gene

    A gene that protects a cell from cancer; loss of function can lead to tumor development.

  • Carcinogen

    A substance or agent that can cause cancer by inducing mutations or promoting cell proliferation.

  • Gene cloning

    The process of making identical copies of a gene or DNA segment.

  • Recombinant DNA

    DNA molecules formed by combining DNA from different sources.

  • Restriction enzyme

    An enzyme that cuts DNA at specific sequences, used in genetic engineering.

  • Sticky ends

    Single-stranded overhangs created by restriction enzymes that facilitate DNA fragment joining.

  • Nucleic acid probe

    A labeled single-stranded DNA or RNA used to detect complementary sequences.

  • cDNA

    Complementary DNA synthesized from an mRNA template using reverse transcriptase.

  • CRISPR-Cas9

    A genome editing tool that allows precise modification of DNA sequences.

  • Genetically modified organism

    An organism whose genome has been altered by genetic engineering techniques.

  • Transgenic organism

    An organism that contains genes from another species introduced by genetic engineering.

  • Gene therapy

    A technique to treat genetic disorders by inserting functional genes into a patient's cells.

  • DNA profiling

    A method to identify individuals based on unique patterns in their DNA.

  • Polymerase chain reaction

    A technique to amplify specific DNA sequences exponentially using primers and DNA polymerase.

  • Primer

    A short DNA sequence that provides a starting point for DNA synthesis in PCR.

  • Gel electrophoresis

    A method to separate DNA fragments by size using an electric field in a gel matrix.

  • Short tandem repeats

    Repeating sequences of 2-5 base pairs in DNA used in DNA profiling.

  • Whole-genome shotgun method

    A sequencing approach that breaks the genome into small fragments, sequences them, and assembles the data.

  • Bioinformatics

    The use of computational tools to analyze and interpret biological data, especially genetic sequences.

  • Proteomics

    The large-scale study of proteins, their structures, and functions.