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Plant Propagation, Biotechnology, and GMOs

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Terms in this set (16)

  • What is recombinant DNA?

    Recombinant DNA is DNA created by combining genetic material from different sources.

  • What is the first step in creating a genetically modified plant?

    Identify the gene for the desired trait.

  • What is done after identifying the gene in recombinant DNA technology?

    Isolate the gene and combine it with a DNA vector.

  • How is DNA inserted into plant cells during genetic modification?

    DNA is inserted into plant cells using a delivery method such as electroporation, particle bombardment, viruses, or bacterial cells.

  • What is the purpose of selecting transformed cells in genetic modification?

    To identify and keep cells that have successfully received the new gene.

  • What happens after selecting transformed plant cells?

    The cells are grown into a whole plant through regeneration.

  • Why is testing important after growing genetically modified plants?

    To confirm that the new gene is working and expressing the desired trait.

  • Describe the electroporation method of DNA delivery.

    Electroporation uses electrical pulses to create temporary openings in cell membranes, allowing DNA to enter.

  • How does particle bombardment deliver DNA into plant cells?

    Tiny metal particles coated with DNA are shot into plant cells to deliver genetic material.

  • What role do viruses play in DNA delivery for genetic modification?

    Modified viruses are used to deliver genetic material into plant cells.

  • How do bacterial cells assist in plant genetic modification?

    Agrobacterium transfers desired DNA into plant cells naturally.

  • Summarize the key steps in recombinant DNA technology for plants.

    Gene → Vector → Delivery → Selection → Regeneration → Testing

  • What is the main goal of producing genetically modified plants?

    To produce plants with desired traits like insect resistance or improved nutrition.

  • How do genetically modified organisms (GMOs) produce new proteins?

    By inserting a gene into an organism, it produces proteins through transcription and translation.

  • Name one benefit of genetically modified organisms (GMOs).

    GMOs can have improved traits such as increased insect resistance or enhanced nutritional value.

  • Name one potential negative aspect of producing GMOs.

    Concerns include ecological impacts, gene flow to wild species, and ethical considerations.