A researcher uses different restriction enzymes to cut the DNA segment and plasmid. What is the likely outcome?

• A. The DNA segment will be degraded by the host cell.
• B. The DNA segment will integrate into the plasmid more efficiently.
• C. The plasmid will replicate the DNA segment more rapidly.
• D. The ends of the DNA segment and plasmid may not be compatible for ligation.

Answer: (D)