Which experimental design would best utilize cDNA libraries to study gene expression changes due to temperature shifts?

• A. Use restriction enzymes to cut mRNA, insert into vectors, sequence to identify expressed genes.
• B. Expose cells to different temperatures, isolate mRNA, create cDNA libraries, sequence to identify expressed genes.
• C. Expose cells to different temperatures, isolate genomic DNA, create genomic libraries, sequence to identify expressed genes.
• D. Expose cells to different temperatures, isolate proteins, create cDNA libraries, sequence to identify expressed genes.

Answer: (B)