In a eukaryotic cell, what would happen if the initiator tRNA failed to bind methionine during translation initiation?

• A. Translation would proceed normally.
• B. The ribosome would not be able to locate the start codon.
• C. The ribosome would skip the first codon and start at the second codon.
• D. Translation would not initiate, as methionine is required to start protein synthesis.

Answer: (D)