Q: Which formula correctly calculates the total magnification of a microscope?

$$\text{Total Magnification} = \text{Ocular Magnification} \times \text{Objective Magnification}$$

Q: If the field diameter under low power (10x objective) is 4 mm, what is the field diameter under high power (40x objective)?

$$4 \text{ mm} \times \dfrac{10}{40} = 1 \text{ mm}$$

Question 1

Which part of the compound microscope is responsible for adjusting the amount of light that reaches the specimen?

Accepted Answer

Iris diaphragm

Question 2

If you are using a 10x ocular lens and a 40x objective lens, what is the total magnification of the specimen?

Accepted Answer

$$10 	imes 40 = 400$$

Question 3

Which of the following best describes the term 'parfocal' in microscopy?

Accepted Answer

The microscope remains in focus when switching between objective lenses

Question 4

A student observes a specimen under low power and then switches to high power. What happens to the field diameter?

Accepted Answer

It decreases

Question 5

Which formula correctly calculates the total magnification of a microscope?

Accepted Answer

$$	ext{Total Magnification} = 	ext{Ocular Magnification} 	imes 	ext{Objective Magnification}$$

Question 6

When using the coarse adjustment knob, which objective lens should you always start with to avoid damaging the slide?

Accepted Answer

Scanning objective

Question 7

If the field diameter under low power (10x objective) is 4 mm, what is the field diameter under high power (40x objective)?

Accepted Answer

$$4 	ext{ mm} 	imes \dfrac{10}{40} = 1 	ext{ mm}$$

Question 8

Which of the following best explains why resolution is important in microscopy?

Accepted Answer

It allows you to distinguish two close objects as separate

Question 9

A student moves the slide to the left while viewing through the microscope. In which direction does the image appear to move?

Accepted Answer

To the right

Question 10

Why is it important to center your object in the field of view before switching to a higher power objective?

Accepted Answer

The field of view decreases and the object may move out of view

Question 11

Which of the following is the correct order of light passage in a compound microscope?

Accepted Answer

Light source → Specimen → Objective lens → Ocular lens

Question 12

If the working distance for a 40x objective is 0.5 mm and for a 10x objective is 4 mm, what does this indicate about working distance and magnification?

Accepted Answer

Working distance decreases as magnification increases

Question 13

A student is using oil immersion objective (100x) with a 10x ocular lens. What is the total magnification?

Accepted Answer

$$100 	imes 10 = 1000$$

Question 14

Which adjustment knob should be used for fine focusing, especially under high power or oil immersion objectives?

Accepted Answer

Fine adjustment knob

Question 15

If the resolution of a microscope is $$0.2 \mu m$$, what does this mean?

Accepted Answer

Objects closer than $$0.2 \mu m$$ appear as one

Question 16

Which of the following is a correct safety procedure when handling a compound microscope?

Accepted Answer

Always use both hands: one on the arm and one supporting the base

Question 17

A student observes that the image is blurry when switching from low power to high power. What is the most likely cause?

Accepted Answer

The microscope is not parfocal

Question 18

Which of the following best describes the relationship between magnification and resolution?

Accepted Answer

Resolution must increase to distinguish details at higher magnification

Question 19

If the field diameter under scanning objective (4x) is 5 mm, what is the field diameter under low power (10x)?

Accepted Answer

$$5 	ext{ mm} 	imes \dfrac{4}{10} = 2 	ext{ mm}$$

Question 20

Why is oil used with the oil immersion objective?

Accepted Answer

To reduce light refraction and improve resolution

Microscopy in Anatomy & Physiology: Principles, Techniques, and Applications

Guida di studio - Domande di pratica

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