Question 1

Which of the following best describes the net energy yield from glycolysis per molecule of glucose?

Accepted Answer

Two ATP and two NADH

Question 2

During glycolysis, what is the primary purpose of the energy investment phase?

Accepted Answer

To provide activation energy by investing ATP

Question 3

Which molecule is the direct product of the energy investment phase of glycolysis that continues into the energy payoff phase?

Accepted Answer

Glyceraldehyde 3-phosphate

Question 4

If a mutation prevented the formation of NADH during glycolysis, which of the following would most directly be affected?

Accepted Answer

The transfer of electrons to the electron transport chain

Question 5

Which of the following statements about the fate of carbon atoms from glucose during glycolysis and pyruvate oxidation is correct?

Accepted Answer

No carbons are released as CO$$_2$$ during glycolysis; the first CO$$_2 is $$released during pyruvate oxidation.

Question 6

A patient’s cells are unable to transport pyruvate into the mitochondria. Which process would be directly impaired?

Accepted Answer

Pyruvate oxidation

Question 7

How many molecules of NADH are produced from one molecule of glucose during glycolysis?

Accepted Answer

Two

Question 8

Which of the following best explains why the net gain of ATP in glycolysis is two, even though four ATP are produced?

Accepted Answer

Two ATP are used during the energy investment phase.

Question 9

During the oxidation of pyruvate, which of the following occurs for each pyruvate molecule?

Accepted Answer

One molecule of CO$$_2 is $$released, one NADH is produced, and one acetyl-CoA is formed.

Question 10

Which of the following is TRUE regarding the location of glycolysis and pyruvate oxidation in eukaryotic cells?

Accepted Answer

Glycolysis occurs in the cytosol, while pyruvate oxidation occurs in the mitochondria.

Question 11

A researcher adds a chemical that blocks substrate-level phosphorylation during glycolysis. What is the most immediate effect on the cell’s energy production?

Accepted Answer

The cell will not generate ATP directly from glycolysis.

Question 12

Which of the following best summarizes the products of glycolysis per molecule of glucose?

Accepted Answer

2 pyruvate, 2 NADH, 2 net ATP, and water

Question 13

Why is most of the energy from glucose still stored in pyruvate at the end of glycolysis?

Accepted Answer

Because only a small fraction of glucose’s covalent bonds are broken during glycolysis.

Question 14

If a cell is deprived of oxygen, which of the following processes can still occur?

Accepted Answer

Glycolysis

Question 15

Which of the following is the correct sequence of events during the early stages of cellular respiration?

Accepted Answer

Glycolysis → Pyruvate oxidation → Citric acid cycle → Oxidative phosphorylation

Question 16

A toxin inhibits the enzyme that converts pyruvate to acetyl-CoA. What is the most immediate consequence for cellular respiration?

Accepted Answer

The citric acid cycle will slow down due to lack of acetyl-CoA.

Question 17

Which of the following equations best represents the net ATP yield from glycolysis per glucose molecule?

Accepted Answer

$$	ext{Net ATP} = 	ext{ATP produced} - 	ext{ATP invested} = 4 - 2 = 2$$

Question 18

Which of the following is a direct product of pyruvate oxidation that enters the citric acid cycle?

Accepted Answer

Acetyl-CoA

Question 19

In a metabolic disorder where the enzyme for substrate-level phosphorylation in glycolysis is defective, which of the following would be most directly affected?

Accepted Answer

ATP production in the cytosol

Question 20

Which of the following best explains why glycolysis is considered an ancient metabolic pathway?

Accepted Answer

It occurs in the cytosol and does not require oxygen.

Cellular Respiration: Glycolysis and Pyruvate Oxidation

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