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General Biology Study Notes: Nutrition, Metabolism, Cellular Respiration, and Photosynthesis

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Nutrition and Homeostasis

Importance of Nutrition in Maintaining Homeostasis

Nutrition is essential for the survival, growth, development, tissue repair, and reproduction of animals. It provides the necessary substances for energy production, cellular function, and the synthesis of new molecules, all of which contribute to the maintenance of homeostasis.

  • Homeostasis: The stable internal environment maintained by living organisms.

  • Nutrients: Substances required for energy, growth, and cellular repair.

  • Essential nutrients: Nutrients that cannot be synthesized by the animal and must be obtained from the diet (e.g., certain amino acids, fatty acids, vitamins, minerals).

Major Categories of Nutrients

Animals consume various nutrients, each with specific functions. These nutrients are classified as organic or inorganic.

Category

Type

General Functions

Carbohydrates

Organic

Primary energy source; component of some proteins; source of carbon.

Proteins

Organic

Provide amino acids for new protein synthesis; build muscle; some used as an energy source.

Lipids

Organic

Major component of cell membranes; energy source; thermal insulator; building blocks of some hormones.

Vitamins

Organic

Important coenzymes. Water-soluble vitamins are not stored, while fat-soluble vitamins are stored in adipose tissue.

Water

Inorganic

Essential for life; acts as a solvent and medium for biochemical reactions.

Minerals

Inorganic

Serve as cofactors/constituents of enzymes; critical for bone, muscle, and nervous system function; contribute to electrical differences across plasma membranes.

Food Processing in Animals

Phases of Food Processing

Food processing in animals occurs in four main phases:

  1. Ingestion: Intake of food.

  2. Digestion: Breakdown of food into smaller molecules.

  3. Absorption: Uptake of nutrients by cells.

  4. Elimination: Removal of undigested material and waste.

Metabolism

Definition and Overview

Metabolism is the sum total of all chemical reactions that occur within an organism. It includes both energy-releasing and energy-consuming processes.

  • Catabolic pathways: Break down molecules to release energy (e.g., glycolysis).

  • Anabolic pathways: Build complex molecules from simpler ones, requiring energy (e.g., protein synthesis).

Energy and Thermodynamics

Energy transformations in metabolism are governed by the laws of thermodynamics:

  • First Law: Energy cannot be created or destroyed, only transformed.

  • Second Law: Every energy transfer increases the entropy (disorder) of the universe.

Chemical potential energy is stored in the electrons of molecules such as glucose and ATP.

Free Energy Change and Chemical Reactions

The change in free energy (ΔG) determines whether a reaction is spontaneous:

  • Exergonic reactions: Release energy; spontaneous; ΔG is negative.

  • Endergonic reactions: Require energy input; not spontaneous; ΔG is positive.

ATP hydrolysis is a key exergonic reaction:

Enzymes and Reaction Rates

Enzymes are biological catalysts that speed up chemical reactions by lowering activation energy.

  • Substrates: Reactants that bind to the enzyme's active site.

  • Induced fit: Enzyme changes shape to better fit the substrate.

  • Inhibitors: Molecules that decrease enzyme activity (competitive and noncompetitive).

  • Cofactors and coenzymes: Non-protein molecules required for enzyme function (e.g., vitamins).

Cellular Respiration

Overview and Stages

Cellular respiration is the process by which cells obtain energy from organic molecules, primarily glucose. It consists of several stages:

Stage

Location

Process Overview

Reactants / Products (Per Glucose)

Glycolysis

Cytosol

Glucose is broken down into two pyruvate via 10 steps. ATP is generated by substrate-level phosphorylation.

Reactants: Glucose, ADP, Stx(NAD)+ Products: 2 Pyruvate, 2 ATP, 2 NADH

Breakdown of Pyruvate

Matrix

Pyruvate is oxidized by pyruvate dehydrogenase; CO2 is removed, and the remaining acetyl group attaches to CoA, forming acetyl CoA.

Reactants: Pyruvate, NAD+, CoA Products: 2 Acetyl CoA, 2 CO2, 2 NADH

Citric Acid Cycle (Krebs)

Mitochondrial Matrix

Acetyl is incorporated into oxaloacetate to form citrate. A series of steps oxidizes the acetyl group, liberating CO2 and regenerating oxaloacetate.

Reactants: 2 Acetyl CoA Products: 4 CO2, 2 ATP (or GTP), 6 NADH, 2 FADH2

Phosphorylation (Electron Transport Chain & Chemiosmosis)

Mitochondrial Membrane

NADH and FADH2 donate electrons to the Electron Transport Chain (ETC). Oxygen is the final electron acceptor. Energy released pumps H+ into the intermembrane space, creating an electrochemical gradient. Chemiosmosis uses this gradient to synthesize ATP from ADP and Pi.

Reactants: NADH, FADH2, O2, ADP, Pi Products: NAD+, FAD, H2O, 30-34 ATP

Fermentation

Fermentation occurs in the absence of oxygen (anaerobic conditions) and allows ATP production via substrate-level phosphorylation.

  • Lactic Acid Fermentation: Muscle cells reduce pyruvate to lactate to regenerate NAD+.

  • Alcohol Fermentation: Yeast cells convert pyruvate to ethanol and CO2.

Catabolism vs. Biosynthesis

Catabolic pathways break down molecules to release energy, while anabolic pathways use energy to synthesize large molecules. The citric acid cycle is central to both, as it can break down or synthesize various nutrient molecules.

Photosynthesis

Overview and Importance

Photosynthesis captures energy from light and uses it to synthesize organic molecules (like glucose) from inorganic molecules (CO2 and H2O). It is central to the energy cycle of life, providing the energy source for most organisms.

Location and Components

  • Occurs in chloroplasts in plants and algae.

  • Main photosynthetic cells are in the mesophyll of leaves.

  • Thylakoid membranes contain the light-absorbing pigments.

  • Calvin Cycle takes place in the stroma (fluid region surrounding thylakoids).

Stages of Photosynthesis

  • Light Reactions: Occur in the thylakoid membrane; convert light energy to chemical energy (ATP and NADPH).

  • Calvin Cycle: Occurs in the stroma; uses ATP and NADPH to fix CO2 into carbohydrates.

Light Absorption and Pigments

  • Chlorophyll: Main pigment absorbing light energy.

  • Shorter wavelengths carry more energy; plants absorb light mainly in the blue and red regions.

Electron Flow in Photosynthesis

  • Linear Electron Flow: Electrons move from water through Photosystem II (PSII) and Photosystem I (PSI) to NADP+, producing both ATP and NADPH.

  • Cyclic Electron Flow: Electrons cycle back to PSI, producing only ATP.

Comparison of ATP and NADPH Production

  • ATP is produced by chemiosmosis in both cellular respiration and photosynthesis.

  • NADPH is produced in the light reactions and used in the Calvin Cycle for carbon fixation.

Summary Table: Photosynthesis vs. Cellular Respiration

Process

Main Function

Location

Key Products

Photosynthesis

Converts light energy to chemical energy

Chloroplasts

Glucose, O2

Cellular Respiration

Converts chemical energy to ATP

Mitochondria

ATP, CO2, H2O

Key Equations

  • Photosynthesis:

  • Cellular Respiration:

Additional info: Some explanations and details have been expanded for clarity and completeness based on standard biology curriculum.

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