Q: Which equation best represents the relationship between DNA methylation and gene expression?

$$\text{Gene Expression} \propto \frac{1}{\text{DNA Methylation}}$$

Question 1

Which of the following best describes the difference between cis-regulation and trans-regulation of gene expression?

Accepted Answer

Cis-regulation involves regulatory elements on the same chromosome as the target gene, while trans-regulation involves factors that can act on genes located on different chromosomes.

Question 2

Which modification to histones is most likely to increase transcriptional activity of a gene?

Accepted Answer

Acetylation of histone tails

Question 3

A transcription factor binds most strongly to the consensus sequence $$5'-TYGCNTCA-3'. $$Which of the following sequences is most likely to be bound by this transcription factor?

Accepted Answer

$$5'-TTGCGTCA-3'$$

Question 4

What is the expected effect of increased DNA methylation at a gene's promoter region?

Accepted Answer

Decreased transcription of the gene

Question 5

Which of the following is an example of post-transcriptional regulation?

Accepted Answer

Alternative splicing of mRNA

Question 6

If a mutation causes a transcription factor to lose its ability to bind DNA, which type of gene regulation is most directly affected?

Accepted Answer

Transcriptional regulation

Question 7

Which of the following statements about epigenetics is TRUE?

Accepted Answer

Epigenetic modifications can be influenced by environmental factors.

Question 8

A mouse with an unmethylated Agouti gene promoter is most likely to have which phenotype?

Accepted Answer

Yellow fur due to increased ASIP expression

Question 9

Which of the following best explains why histone acetylation leads to increased gene expression?

Accepted Answer

Acetylation reduces the positive charge of histones, causing DNA to bind less tightly and increasing accessibility for transcription factors.

Question 10

Which of the following scenarios best illustrates phenotypic plasticity due to epigenetic modification?

Accepted Answer

A plant changes leaf shape in response to sunlight, mediated by changes in DNA methylation patterns.

Question 11

Which of the following is NOT a mechanism of gene expression regulation?

Accepted Answer

Genetic recombination

Question 12

A SNP in the intron of a gene alters the binding site for a histone acetylase. What is the most likely consequence?

Accepted Answer

Altered acetylation pattern, potentially changing gene expression

Question 13

Which equation best represents the relationship between DNA methylation and gene expression?

Accepted Answer

$$	ext{Gene Expression} \propto \frac{1}{	ext{DNA Methylation}}$$

Question 14

Which of the following is most likely to occur if maternal diet is supplemented with methyl donors during pregnancy in mice?

Accepted Answer

Offspring with methylated Agouti gene and typical brown phenotype

Question 15

Which of the following is a direct effect of DNA methylation at a gene promoter?

Accepted Answer

Recruitment of histone deacetylases (HDAC)

Question 16

Which of the following statements about consensus sequences is correct?

Accepted Answer

Consensus sequences represent the most common base at each position among a set of sequences.

Question 17

Which of the following environmental factors is most likely to influence epigenetic regulation of gene expression?

Accepted Answer

Exposure to folate-rich foods

Question 18

Which process is most directly affected by the packaging of DNA into heterochromatin?

Accepted Answer

Transcription

Question 19

Which of the following is a plausible consequence of increased histone acetylation in cancer cells?

Accepted Answer

Increased gene expression in oncogenes

Question 20

Which of the following best describes the role of transcription factors in gene regulation?

Accepted Answer

Transcription factors bind to DNA and regulate the rate of transcription initiation.

Gene Expression and Epigenetic Regulation: Mechanisms and Impacts

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