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Comprehensive Study Guide for Microbiology: Metabolism, Nutrition, Genetics, and Biotechnology

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Introduction to Metabolism

  • Introduction to Metabolism
    02:17
  • Catabolic & Anabolic Pathways
    03:25

ATP

  • Energy Coupling
    04:57
  • ATP
    06:44
  • Phosphorylation
    03:00

Types of Phosphorylation

  • Substrate-Level Phosphorylation
    03:00
  • Oxidative Phosphorylation
    06:27
  • Types of Phosphorylation
    01:06

Redox Reactions

  • Redox Reactions Example 1
    01:44
  • Electron Carriers: NADH & FADH2
    05:22
  • Redox Reactions
    04:46

Enzymes

  • Functions of Enzymes
    02:32
  • Enzymes Example 1
    02:25
  • Enzymes
    02:55

Enzyme Activation Energy

  • Enzyme Activation Energy
    04:18
  • Enzymes Lower EA
    03:26

Enzyme Binding Factors

  • Cofactors
    04:22
  • Enzyme-Substrate Complex
    03:49

Enzyme Inhibition

  • Enzyme Inhibition
    05:03

Introduction to Aerobic Cellular Respiration

  • Stages of Aerobic Cellular Respiration
    02:32
  • Introduction to Aerobic Cellular Respiration
    03:38
  • Introduction to Aerobic Cellular Respiration Example 2
    01:53

Glycolysis

  • Phases of Glycolysis
    05:57
  • Glycolysis Example 1
    01:29
  • Remembering Phases of Glycolysis
    02:49

Krebs Cycle

  • Krebs Cycle Example 1
    01:20
  • Krebs Cycle
    01:37
  • Phases of The Krebs Cycle
    09:54

Electron Transport Chain

  • Remembering the ETC
    04:43
  • Electron Transport Chain in Prokaryotes
    02:49
  • Electron Transport Chain
    07:41

Chemiosmosis

  • Chemiosmosis
    05:01

Fermentation & Anaerobic Respiration

  • Lactic Acid Fermentation
    03:33
  • What Happens to Aerobic Organisms if There's No Oxygen?
    07:50
  • Alcohol Fermentation
    01:59

Introduction to Photosynthesis

  • Introduction to Photosynthesis
    03:32
  • Photosynthesis is a Redox Reaction
    03:20
  • Photosynthesis vs. Cellular Respiration
    07:55

Nutritional Factors of Microbial Growth

  • Reviewing the Nutritional Growth Factors of Microbes
    02:20
  • Carbon Source:Heterotrophs vs. Autotrophs
    02:59
  • Nutritional Factors of Microbial Growth
    02:57

Oxygen Requirements for Microbial Growth

  • Reactive Oxygen Species
    04:43
  • Oxygen Requirement Classes of Microbes
    07:27
  • Oxygen Requirements for Microbial Growth
    03:52

Temperature Requirements for Microbial Growth

  • Temperature Requirements for Microbial Growth
    04:16
  • Classification of Microbes by Growth Temperature
    04:56
  • How to Memorize the Classification of Microbes by Growth Temperatures
    01:59

Osmolarity Factors for Microbial Growth

  • Osmolarity Factors for Microbial Growth
    06:30

Biofilms

  • Quorum Sensing
    04:13
  • Steps of Biofilm Development
    04:32
  • Biofilms
    03:14

Introduction to Cultivating Microbial Growth

  • Map of Techniques for Cultivating Microbes
    02:05
  • Introduction to Cultivating Microbial Growth
    03:49

Growing a Pure Culture

  • Growing a Pure Culture
    02:58

Types of Solid Culture Media

  • Types of Solid Culture Media
    03:16

Introduction to the Types of Culture Media

  • Introduction to the Types of Culture Media
    02:42

Chemically Defined Media

  • Chemically Defined Media
    01:53

Complex Media

  • Complex Media
    02:59

Selective Media

  • Selective Media
    03:27

Enrichment Media

  • Enrichment Media
    04:20

Differential Media

  • Differential Media
    04:34

Microbial Growth Curves in a Closed System

  • Microbial Growth Curves in a Closed System
    08:59
  • Primary & Secondary Metabolites
    02:33

Generation Times

  • Calculating Generation Times
    05:48
  • Generation Times
    02:54

Measuring Growth by Plate Counts

  • Measuring Growth by Plate Counts
    07:16
  • Measuring Growth by Plate Counts
    01:36

Measuring Growth by Membrane Filtration

  • Measuring Growth by Membrane Filtration
    02:29

Measuring Growth by Direct Cell Counts

  • Types of Direct Cell Counting Instruments
    02:32
  • Direct Microscopic Cell Counts
    03:28

Introduction to Microbial Genetics

  • Map of the Lesson on Microbial Genetics
    02:04
  • Introduction to Microbial Genetics
    04:20

Introduction to DNA Replication

  • Unwinding the DNA:Topoisomerase, Helicase & SSBs
    05:10
  • Components of DNA Replication
    04:47
  • Origin of Replication
    03:33

Steps of DNA Replication

  • Steps of DNA Replication
    09:29

Central Dogma

  • Central Dogma
    05:45

Introduction to Transcription

  • Overview of Transcription
    07:44
  • Introduction to Transcription
    05:51

Steps of Transcription

  • Elongation of Transcription
    02:44
  • Initiation of Transcription
    04:16
  • Elongation of Transcription
    04:01

Genetic Code

  • How to Use the Genetic Code
    11:39
  • Genetic Code
    01:28

Introduction to Types of RNA

  • Introduction to Types of RNA
    07:20

Introduction to Translation

  • Ribosomal tRNA Binding Sites
    06:16
  • Ribosome Subunits
    08:42
  • Introduction to Translation
    06:12

Steps of Translation

  • Elongation of Translation
    04:59
  • Termination of Translation
    02:16
  • Steps of Translation
    05:03

Introduction to Mutations

  • Spontaneous vs. Induced Mutations
    02:47
  • Introduction to Mutations
    00:37
  • Types of Mutations
    08:44

Horizontal Gene Transfer

  • Fates of Horizontally Transferred DNA
    03:13
  • Horizontal Gene Transfer
    04:42
  • Integration of DNA via Homologous Recombination
    02:52

Transduction

  • Generalized Transduction
    04:24
  • Transduction
    06:49
  • Specialized Transduction
    06:34

Introduction to Conjugation

  • Introduction to Conjugation
    04:21

Conjugation: F Plasmids

  • F Plasmids
    05:57
  • Mechanism of F Plasmid Conjugation in E. coli
    04:12

Introduction to DNA-Based Technology

  • Map of DNA-Based Technology Lesson
    03:12
  • Introduction to DNA-Based Technology
    02:30

Introduction to Polymerase Chain Reaction

  • Introduction to Polymerase Chain Reaction
    01:31
  • Components of a Polymerase Chain Reaction
    05:57
  • Why do we use PCR?
    02:53

The Steps of PCR

  • 1) Denaturation
    03:33
  • 2) Annealing
    02:56
  • The Steps of PCR
    04:39

Introduction to DNA Cloning

  • Introduction to DNA Cloning
    02:25
  • Cloning with Recombinant DNA
    03:41

Steps to DNA Cloning

  • Steps to DNA Cloning
    04:42
  • 1b) Use Ligation Enzymes
    04:58
  • 1a) Use Restriction Enzymes
    03:27

Gel Electrophoresis

  • Gel Electrophoresis
    07:58
  • Gel Electrophoresis Example 1
    02:34

Southern Blotting

  • Southern Blotting
    03:31
  • Steps of Southern Blotting
    08:21

Introduction to DNA Sequencing

  • Introduction to DNA Sequencing
    02:13
  • Dideoxy Nucleotides
    05:12

Dideoxy Sequencing

  • Chain-Termination PCR
    07:37
  • Dideoxy Sequencing
    01:03
  • Determining the DNA Sequence from a Gel
    06:28