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MCB2000 Exam Two Study Guide – Step-by-Step Microbiology Guidance

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Central Dogma

  • Central Dogma
    05:45

Introduction to Types of RNA

  • Introduction to Types of RNA
    07:20

Introduction to DNA Replication

  • Unwinding the DNA:Topoisomerase, Helicase & SSBs
    05:10
  • Components of DNA Replication
    04:47
  • Origin of Replication
    03:33

Steps of DNA Replication

  • Steps of DNA Replication
    09:29

Introduction to Transcription

  • Overview of Transcription
    07:44
  • Introduction to Transcription
    05:51

Steps of Transcription

  • Elongation of Transcription
    02:44
  • Initiation of Transcription
    04:16
  • Elongation of Transcription
    04:01

Introduction to Translation

  • Ribosomal tRNA Binding Sites
    06:16
  • Ribosome Subunits
    08:42
  • Introduction to Translation
    06:12

Prokaryotic Gene Expression

  • Location of Transcription & Translation
    03:17
  • Simultaneous Transcription & Translation in Prokaryotes
    03:34
  • Prokaryotic Sigma (σ) Factors
    05:00

Introduction to Regulation of Gene Expression

  • Positive vs Negative Gene Regulation
    02:17
  • Introduction to Regulation of Gene Expression
    06:40

Prokaryotic Gene Regulation via Operons

  • Inducible Operons
    04:25
  • Prokaryotic Gene Regulation via Operons
    00:53
  • Repressible Operons
    03:42

The Lac Operon

  • In the Absence of Lactose
    04:34
  • The Lac Operon
    07:06
  • In the Presence of Lactose
    03:56

Introduction to Mutations

  • Spontaneous vs. Induced Mutations
    02:47
  • Introduction to Mutations
    00:37
  • Types of Mutations
    08:44

Methods of Inducing Mutations

  • Chemical Mutagens
    05:13
  • Radiation Mutagens
    02:55
  • Methods of Inducing Mutations
    03:19

Mutant Detection

  • Mutant Detection
    06:49
  • Replica Plating
    05:36

The Ames Test

  • The Ames Test
    06:59

Introduction to Microbial Genetics

  • Map of the Lesson on Microbial Genetics
    02:04
  • Introduction to Microbial Genetics
    04:20

Horizontal Gene Transfer

  • Fates of Horizontally Transferred DNA
    03:13
  • Horizontal Gene Transfer
    04:42
  • Integration of DNA via Homologous Recombination
    02:52

Bacterial Transformation

  • Bacterial Transformation
    03:06
  • Mechanism of Natural Transformation
    03:49

Introduction to Conjugation

  • Introduction to Conjugation
    04:21

Conjugation: F Plasmids

  • F Plasmids
    05:57
  • Mechanism of F Plasmid Conjugation in E. coli
    04:12

Conjugation: Hfr & F' Cells

  • F’ Cell Conjugation
    04:31
  • Hfr Cell Conjugation
    09:41

Transduction

  • Generalized Transduction
    04:24
  • Transduction
    06:49
  • Specialized Transduction
    06:34

Introduction to DNA-Based Technology

  • Map of DNA-Based Technology Lesson
    03:12
  • Introduction to DNA-Based Technology
    02:30

Introduction to Polymerase Chain Reaction

  • Introduction to Polymerase Chain Reaction
    01:31
  • Components of a Polymerase Chain Reaction
    05:57
  • Why do we use PCR?
    02:53

The Steps of PCR

  • 1) Denaturation
    03:33
  • 2) Annealing
    02:56
  • The Steps of PCR
    04:39

Introduction to DNA Cloning

  • Introduction to DNA Cloning
    02:25
  • Cloning with Recombinant DNA
    03:41

Steps to DNA Cloning

  • Steps to DNA Cloning
    04:42
  • 1b) Use Ligation Enzymes
    04:58
  • 1a) Use Restriction Enzymes
    03:27

Introduction to Viruses

  • General Structure of Viruses
    04:47
  • Three Common Shapes of Viruses
    03:19
  • Enveloped vs. Non-Enveloped Viruses
    05:09

Plaque Assays

  • Plaque Assays
    04:21

Introduction to Animal Virus Infections

  • Introduction to Animal Virus Infections
    02:33
  • Memorizing the Steps of Animal Virus Infections
    01:40

Bacteriophage: Lytic Phage Infections

  • Bacteriophage: Lytic Phage Infections
    04:58

Bacteriophage: Lysogenic Phage Infections

  • Bacteriophage: Lysogenic Phage Infections
    05:27
  • Lysogenic Conversion
    03:37

Animal Viruses: 1. Attachment to the Host Cell

  • Animal Viruses: 1. Attachment to the Host Cell
    03:12

Animal Viruses: 2. Entry & Uncoating in the Host Cell

  • Entry & Uncoating by Endocytosis
    02:29
  • Animal Viruses: 2. Entry & Uncoating in the Host Cell
    04:29
  • Entry & Uncoating of Enveloped Viruses by Membrane Fusion
    02:59

Animal Viruses: 3. Synthesis & Replication

  • Map of Lesson on Animal Virus Synthesis & Replication
    03:34
  • Types of RNA Virus Genomes
    10:46
  • Common Themes of All Virus Genomes
    02:58

Animal Viruses: DNA Virus Synthesis & Replication

  • Animal Viruses: DNA Virus Synthesis & Replication
    01:10
  • Replication of Single-Stranded DNA (ssDNA) Viruses
    04:12
  • Replication of Double-Stranded DNA (dsDNA) Viruses
    03:58

Animal Viruses: RNA Virus Synthesis & Replication

  • Animal Viruses: RNA Virus Synthesis & Replication
    01:30
  • Replication of (+) Single Strand RNA (ssRNA) Viruses
    03:52
  • Replication of Double-Stranded RNA Viruses
    04:28

Acute vs. Persistent Viral Infections

  • Acute Viral Infections
    03:29
  • Persistent Viral Infections
    05:12
  • Acute vs. Persistent Viral Infections
    02:59

The Human Microbiome

  • The Human Microbiome
    03:25
  • Microbiome Promotes Immune Tolerance
    05:48
  • Microbiome Makes Nutrients & Aids in Host Digestion
    03:26