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Microbiology Core Concepts: Learning Objectives and Study Guide

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Introduction to Spontaneous Generation

  • Biogenesis vs. Spontaneous Generation
    02:05
  • Introduction to Spontaneous Generation
    05:59

Francesco Redi's Experiments

  • Francesco Redi's Experiments
    05:16

Needham vs. Spallanzani

  • Needham vs. Spallanzani
    05:56
  • Needham vs. Spallanzani Experimental Design
    05:34
  • Spallanzani’s Results Disproved Spontaneous Generation
    07:22

Pasteur's Experiments on Spontaneous Generation

  • Pasteur's Experiments on Spontaneous Generation
    03:50
  • Pasteur’s Swan-Neck Experiment
    05:58

Introduction to Bacteria

  • Introduction to Bacteria
    06:18

Introduction to Microscopes

  • Introduction to Microscopes
    06:13

Magnification, Resolution, & Contrast

  • Magnification, Resolution, & Contrast
    07:29

Gram Stain

  • Gram Stain
    04:57

Bacterial Cell Morphology & Arrangements

  • Types of Bacterial Cell Morphology
    02:44
  • Bacterial Cell Morphology & Arrangements
    01:13
  • Bacilli Bacterial Cell Arrangements
    03:56

Introduction to Bacterial Cell Walls

  • Peptidoglycan
    01:58
  • Types of Bacterial Cell Walls
    02:44
  • Peptidoglycan Structure
    04:44

The Glycocalyx: Capsules & Slime Layers

  • The Glycocalyx: Capsules & Slime Layers
    02:26
  • Capsules vs. Slime Layers
    01:53

Introduction to Biofilms

  • Introduction to Biofilms
    03:31

Pili

  • Sex Pilus
    03:29
  • Cell Motility by Pili
    02:59
  • Pili
    01:02

Fimbriae & Hami

  • Fimbriae & Hami
    01:52
  • Hami
    01:51

Introduction to Prokaryotic Flagella

  • Types of Flagellar Distribution on Bacteria
    05:22
  • Introduction to Prokaryotic Flagella
    02:07

Binary Fission

  • Binary Fission
    02:16
  • Steps of Binary Fission
    04:57

Microbial Growth Curves in a Closed System

  • Microbial Growth Curves in a Closed System
    08:59
  • Primary & Secondary Metabolites
    02:33

Temperature Requirements for Microbial Growth

  • Temperature Requirements for Microbial Growth
    04:16
  • Classification of Microbes by Growth Temperature
    04:56
  • How to Memorize the Classification of Microbes by Growth Temperatures
    01:59

Oxygen Requirements for Microbial Growth

  • Reactive Oxygen Species
    04:43
  • Oxygen Requirement Classes of Microbes
    07:27
  • Oxygen Requirements for Microbial Growth
    03:52

pH Requirements for Microbial Growth

  • pH Requirements for Microbial Growth
    03:11
  • Optimal Growth pH Classes of Microbes
    03:23

Osmolarity Factors for Microbial Growth

  • Osmolarity Factors for Microbial Growth
    06:30

Introduction to Cultivating Microbial Growth

  • Map of Techniques for Cultivating Microbes
    02:05
  • Introduction to Cultivating Microbial Growth
    03:49

Types of Solid Culture Media

  • Types of Solid Culture Media
    03:16

Plating Methods

  • Streak-Plate Method
    05:16
  • Spread-Plate Method vs. Pour-Plate Method
    05:36

Introduction to Controlling Microbial Growth

  • Terminology of Microbial Growth Control
    07:36
  • Hierarchy of Microbial Growth Control Terminology
    03:09
  • Situations Warranting Different Levels of Microbial Growth Control
    05:58

Selecting a Method to Control Microbial Growth

  • Types of Microbes (Microbial Characteristics)
    07:24
  • Overall Risk of Infection
    04:52
  • Environmental Factors
    04:18

Physical Methods to Control Microbial Growth

  • Dry Heat
    02:58
  • Irradiation
    06:18
  • Filtration
    04:29

Chemical Methods to Control Microbial Growth

  • Types of Disinfectants
    05:21
  • Selecting the Right Chemical Agent
    04:52
  • Chemical Methods to Control Microbial Growth
    03:58

The Griffith Experiment

  • The Griffith Experiment
    08:56

The Hershey-Chase Experiment

  • The Hershey-Chase Experiment
    03:59
  • Bacteriophages
    03:44

Chargaff's Rules

  • Chargaff's Rules
    04:33

Discovering the Structure of DNA

  • Detailed DNA Structure
    06:24
  • Discovering the Structure of DNA
    04:41

Meselson-Stahl Experiment

  • Meselson-Stahl Experiment
    09:03

Introduction to DNA Replication

  • Unwinding the DNA:Topoisomerase, Helicase & SSBs
    05:10
  • Components of DNA Replication
    04:47
  • Origin of Replication
    03:33

DNA Polymerases

  • DNA Polymerase Distinguishes Template from New Strand via Methylation
    03:47
  • DNA Polymerases Requirements
    05:03
  • DNA Polymerases
    04:01

Leading & Lagging DNA Strands

  • Leading & Lagging DNA Strands
    05:15

Steps of DNA Replication

  • Steps of DNA Replication
    09:29

DNA Repair

  • DNA Repair
    03:20
  • DNA Proofreading & Repair Enzymes
    02:12

Central Dogma

  • Central Dogma
    05:45

Introduction to Transcription

  • Overview of Transcription
    07:44
  • Introduction to Transcription
    05:51

Steps of Transcription

  • Elongation of Transcription
    02:44
  • Initiation of Transcription
    04:16
  • Elongation of Transcription
    04:01

Transcription Termination in Prokaryotes

  • Transcription Termination in Prokaryotes
    04:17

Eukaryotic RNA Processing and Splicing

  • Eukaryotic RNA Processing and Splicing
    02:32
  • 2) RNA Splicing Creates Mature mRNA
    08:44
  • 1) RNA Processing
    04:19

Introduction to Types of RNA

  • Introduction to Types of RNA
    07:20

Genetic Code

  • How to Use the Genetic Code
    11:39
  • Genetic Code
    01:28

Introduction to Translation

  • Ribosomal tRNA Binding Sites
    06:16
  • Ribosome Subunits
    08:42
  • Introduction to Translation
    06:12

Steps of Translation

  • Elongation of Translation
    04:59
  • Termination of Translation
    02:16
  • Steps of Translation
    05:03

Prokaryotic Gene Expression

  • Location of Transcription & Translation
    03:17
  • Simultaneous Transcription & Translation in Prokaryotes
    03:34
  • Prokaryotic Sigma (σ) Factors
    05:00

Introduction to Regulation of Gene Expression

  • Positive vs Negative Gene Regulation
    02:17
  • Introduction to Regulation of Gene Expression
    06:40

Prokaryotic Gene Regulation via Operons

  • Inducible Operons
    04:25
  • Prokaryotic Gene Regulation via Operons
    00:53
  • Repressible Operons
    03:42

The Lac Operon

  • In the Absence of Lactose
    04:34
  • The Lac Operon
    07:06
  • In the Presence of Lactose
    03:56

Glucose's Impact on Lac Operon

  • Positive Control by cAMP & CRP
    08:32
  • Glucose's Impact on Lac Operon Example 1
    06:20
  • Glucose Levels, cAMP, & the Lac Operon
    05:58

The Trp Operon

  • In the Presence of Tryptophan
    03:11
  • The Trp Operon
    05:30
  • In the Absence of Tryptophan
    03:42

Introduction to Microbial Genetics

  • Map of the Lesson on Microbial Genetics
    02:04
  • Introduction to Microbial Genetics
    04:20

Introduction to Mutations

  • Spontaneous vs. Induced Mutations
    02:47
  • Introduction to Mutations
    00:37
  • Types of Mutations
    08:44

Mutant Detection

  • Mutant Detection
    06:49
  • Replica Plating
    05:36

The Ames Test

  • The Ames Test
    06:59

Introduction to DNA Repair

  • Introduction to DNA Repair
    02:47

DNA Repair Mechanisms

  • Nucleotide Excision Repair
    03:31
  • SOS Repair System
    03:50
  • Photoreactivation
    02:29

Horizontal Gene Transfer

  • Fates of Horizontally Transferred DNA
    03:13
  • Horizontal Gene Transfer
    04:42
  • Integration of DNA via Homologous Recombination
    02:52

Bacterial Transformation

  • Bacterial Transformation
    03:06
  • Mechanism of Natural Transformation
    03:49

Transduction

  • Generalized Transduction
    04:24
  • Transduction
    06:49
  • Specialized Transduction
    06:34

Introduction to Conjugation

  • Introduction to Conjugation
    04:21

Conjugation: F Plasmids

  • F Plasmids
    05:57
  • Mechanism of F Plasmid Conjugation in E. coli
    04:12

Conjugation: Hfr & F' Cells

  • F’ Cell Conjugation
    04:31
  • Hfr Cell Conjugation
    09:41