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Microbiology Study Guide: Genetics, Viruses & Prions, Microbial Growth, and Metabolism (Chapters 5-8)

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Central Dogma

  • Central Dogma
    05:45

Introduction to DNA Replication

  • Unwinding the DNA:Topoisomerase, Helicase & SSBs
    05:10
  • Components of DNA Replication
    04:47
  • Origin of Replication
    03:33

DNA Polymerases

  • DNA Polymerase Distinguishes Template from New Strand via Methylation
    03:47
  • DNA Polymerases Requirements
    05:03
  • DNA Polymerases
    04:01

Leading & Lagging DNA Strands

  • Leading & Lagging DNA Strands
    05:15

Steps of DNA Replication

  • Steps of DNA Replication
    09:29

Introduction to Transcription

  • Overview of Transcription
    07:44
  • Introduction to Transcription
    05:51

Steps of Transcription

  • Elongation of Transcription
    02:44
  • Initiation of Transcription
    04:16
  • Elongation of Transcription
    04:01

Introduction to Types of RNA

  • Introduction to Types of RNA
    07:20

Genetic Code

  • How to Use the Genetic Code
    11:39
  • Genetic Code
    01:28

Introduction to Translation

  • Ribosomal tRNA Binding Sites
    06:16
  • Ribosome Subunits
    08:42
  • Introduction to Translation
    06:12

Steps of Translation

  • Elongation of Translation
    04:59
  • Termination of Translation
    02:16
  • Steps of Translation
    05:03

Review of Transcription vs. Translation

  • Review of Transcription vs. Translation
    04:07

Post-Translational Modification

  • Post-Translational Modification
    04:26

Introduction to Regulation of Gene Expression

  • Positive vs Negative Gene Regulation
    02:17
  • Introduction to Regulation of Gene Expression
    06:40

Eukaryotic Chromatin Modifications

  • Eukaryotic Chromatin Modifications
    03:55
  • Histone Acetylation
    04:14
  • DNA Methylation
    03:39

Prokaryotic Gene Regulation via Operons

  • Inducible Operons
    04:25
  • Prokaryotic Gene Regulation via Operons
    00:53
  • Repressible Operons
    03:42

The Lac Operon

  • In the Absence of Lactose
    04:34
  • The Lac Operon
    07:06
  • In the Presence of Lactose
    03:56

The Trp Operon

  • In the Presence of Tryptophan
    03:11
  • The Trp Operon
    05:30
  • In the Absence of Tryptophan
    03:42

Review of the Lac Operon & Trp Operon

  • Review of the Lac Operon & Trp Operon
    06:24

Eukaryotic Post-Transcriptional Regulation

  • Eukaryotic Post-Transcriptional Regulation
    01:56
  • 3) RNA Interference
    04:16
  • 1) Alternative RNA Splicing
    05:17

Introduction to Mutations

  • Spontaneous vs. Induced Mutations
    02:47
  • Introduction to Mutations
    00:37
  • Types of Mutations
    08:44

Methods of Inducing Mutations

  • Chemical Mutagens
    05:13
  • Radiation Mutagens
    02:55
  • Methods of Inducing Mutations
    03:19

Mutant Detection

  • Mutant Detection
    06:49
  • Replica Plating
    05:36

The Ames Test

  • The Ames Test
    06:59

Introduction to DNA Repair

  • Introduction to DNA Repair
    02:47

DNA Repair Mechanisms

  • Nucleotide Excision Repair
    03:31
  • SOS Repair System
    03:50
  • Photoreactivation
    02:29

Horizontal Gene Transfer

  • Fates of Horizontally Transferred DNA
    03:13
  • Horizontal Gene Transfer
    04:42
  • Integration of DNA via Homologous Recombination
    02:52

Bacterial Transformation

  • Bacterial Transformation
    03:06
  • Mechanism of Natural Transformation
    03:49

Transduction

  • Generalized Transduction
    04:24
  • Transduction
    06:49
  • Specialized Transduction
    06:34

Introduction to Conjugation

  • Introduction to Conjugation
    04:21

Conjugation: F Plasmids

  • F Plasmids
    05:57
  • Mechanism of F Plasmid Conjugation in E. coli
    04:12

Conjugation: Hfr & F' Cells

  • F’ Cell Conjugation
    04:31
  • Hfr Cell Conjugation
    09:41

CRISPR CAS

  • CRISPR CAS
    07:43

Introduction to Viruses

  • General Structure of Viruses
    04:47
  • Three Common Shapes of Viruses
    03:19
  • Enveloped vs. Non-Enveloped Viruses
    05:09

Introduction to Bacteriophage Infections

  • Map of Lesson on Bacteriophage Infections
    03:52
  • Productive vs. Latent State Infections
    04:09
  • Lytic & Lysogenic Bacteriophages
    04:34

Bacteriophage: Lytic Phage Infections

  • Bacteriophage: Lytic Phage Infections
    04:58

Bacteriophage: Lysogenic Phage Infections

  • Bacteriophage: Lysogenic Phage Infections
    05:27
  • Lysogenic Conversion
    03:37

Plaque Assays

  • Plaque Assays
    04:21

Introduction to Animal Virus Infections

  • Introduction to Animal Virus Infections
    02:33
  • Memorizing the Steps of Animal Virus Infections
    01:40

Animal Viruses: 1. Attachment to the Host Cell

  • Animal Viruses: 1. Attachment to the Host Cell
    03:12

Animal Viruses: 2. Entry & Uncoating in the Host Cell

  • Entry & Uncoating by Endocytosis
    02:29
  • Animal Viruses: 2. Entry & Uncoating in the Host Cell
    04:29
  • Entry & Uncoating of Enveloped Viruses by Membrane Fusion
    02:59

Animal Viruses: 3. Synthesis & Replication

  • Map of Lesson on Animal Virus Synthesis & Replication
    03:34
  • Types of RNA Virus Genomes
    10:46
  • Common Themes of All Virus Genomes
    02:58

Animal Viruses: DNA Virus Synthesis & Replication

  • Animal Viruses: DNA Virus Synthesis & Replication
    01:10
  • Replication of Single-Stranded DNA (ssDNA) Viruses
    04:12
  • Replication of Double-Stranded DNA (dsDNA) Viruses
    03:58

Animal Viruses: RNA Virus Synthesis & Replication

  • Animal Viruses: RNA Virus Synthesis & Replication
    01:30
  • Replication of (+) Single Strand RNA (ssRNA) Viruses
    03:52
  • Replication of Double-Stranded RNA Viruses
    04:28

Animal Viruses: Antigenic Drift vs. Antigenic Shift

  • Animal Viruses: Antigenic Drift vs. Antigenic Shift
    05:27

Animal Viruses: Reverse-Transcribing Virus Synthesis & Replication

  • Animal Viruses: Reverse-Transcribing Virus Synthesis & Replication
    04:28

Animal Viruses: 4. Assembly Inside Host Cell

  • Animal Viruses: 4. Assembly Inside Host Cell
    04:26

Animal Viruses: 5. Release from Host Cell

  • Release of Non-Enveloped Viruses
    02:34
  • Release of Enveloped Viruses
    05:08

Acute vs. Persistent Viral Infections

  • Acute Viral Infections
    03:29
  • Persistent Viral Infections
    05:12
  • Acute vs. Persistent Viral Infections
    02:59

Prions

  • Prion Development in Neurons Leads to Scrapie
    04:00
  • Prions
    03:05

Biofilms

  • Quorum Sensing
    04:13
  • Steps of Biofilm Development
    04:32
  • Biofilms
    03:14

Binary Fission

  • Binary Fission
    02:16
  • Steps of Binary Fission
    04:57

Microbial Growth Curves in a Closed System

  • Microbial Growth Curves in a Closed System
    08:59
  • Primary & Secondary Metabolites
    02:33

Temperature Requirements for Microbial Growth

  • Temperature Requirements for Microbial Growth
    04:16
  • Classification of Microbes by Growth Temperature
    04:56
  • How to Memorize the Classification of Microbes by Growth Temperatures
    01:59

Oxygen Requirements for Microbial Growth

  • Reactive Oxygen Species
    04:43
  • Oxygen Requirement Classes of Microbes
    07:27
  • Oxygen Requirements for Microbial Growth
    03:52

pH Requirements for Microbial Growth

  • pH Requirements for Microbial Growth
    03:11
  • Optimal Growth pH Classes of Microbes
    03:23

Osmolarity Factors for Microbial Growth

  • Osmolarity Factors for Microbial Growth
    06:30

Reviewing the Environmental Factors of Microbial Growth

  • Reviewing the Environmental Factors of Microbial Growth
    07:21

Nutritional Factors of Microbial Growth

  • Reviewing the Nutritional Growth Factors of Microbes
    02:20
  • Carbon Source:Heterotrophs vs. Autotrophs
    02:59
  • Nutritional Factors of Microbial Growth
    02:57

Growth Factors

  • Growth Factors
    01:55

Introduction to Cultivating Microbial Growth

  • Map of Techniques for Cultivating Microbes
    02:05
  • Introduction to Cultivating Microbial Growth
    03:49

Types of Solid Culture Media

  • Types of Solid Culture Media
    03:16

Plating Methods

  • Streak-Plate Method
    05:16
  • Spread-Plate Method vs. Pour-Plate Method
    05:36

Introduction to the Types of Culture Media

  • Introduction to the Types of Culture Media
    02:42

Chemically Defined Media

  • Chemically Defined Media
    01:53

Complex Media

  • Complex Media
    02:59

Selective Media

  • Selective Media
    03:27

Differential Media

  • Differential Media
    04:34

Reducing Media

  • Reducing Media
    02:55

Enrichment Media

  • Enrichment Media
    04:20

Measuring Growth by Direct Cell Counts

  • Types of Direct Cell Counting Instruments
    02:32
  • Direct Microscopic Cell Counts
    03:28

Measuring Growth by Plate Counts

  • Measuring Growth by Plate Counts
    07:16
  • Measuring Growth by Plate Counts
    01:36

Measuring Growth by Membrane Filtration

  • Measuring Growth by Membrane Filtration
    02:29

Measuring Growth by Biomass

  • Measuring Total Weight
    03:27
  • Measuring Growth by Biomass
    05:58

Introduction to Controlling Microbial Growth

  • Terminology of Microbial Growth Control
    07:36
  • Hierarchy of Microbial Growth Control Terminology
    03:09
  • Situations Warranting Different Levels of Microbial Growth Control
    05:58

Selecting a Method to Control Microbial Growth

  • Types of Microbes (Microbial Characteristics)
    07:24
  • Overall Risk of Infection
    04:52
  • Environmental Factors
    04:18

Physical Methods to Control Microbial Growth

  • Dry Heat
    02:58
  • Irradiation
    06:18
  • Filtration
    04:29

Chemical Methods to Control Microbial Growth

  • Types of Disinfectants
    05:21
  • Selecting the Right Chemical Agent
    04:52
  • Chemical Methods to Control Microbial Growth
    03:58

Chemicals Used to Control Microbial Growth

  • Chemicals Used to Control Microbial Growth
    02:29
  • Factors Affecting Chemical Agents
    03:17

Liquid Chemicals: Alcohols, Aldehydes, & Biguanides

  • Alcohols
    03:39
  • Biguanides
    02:41
  • Aldehydes
    03:59

Liquid Chemicals: Halogens

  • Iodine
    02:15
  • Chlorine
    03:16
  • Liquid Chemicals: Halogens
    00:56

Liquid Chemicals: Surface-Active Agents

  • Liquid Chemicals: Surface-Active Agents
    04:14
  • Soaps & Detergents
    06:20
  • Quats
    02:33

Chemical Gases: Ethylene Oxide, Ozone, & Formaldehyde

  • Ozone
    02:36
  • Formaldehyde Gas
    02:56
  • Chemical Gases: Ethylene Oxide
    03:22

Review of Chemicals Used to Control Microbial Growth

  • Review of Chemicals Used to Control Microbial Growth
    05:41

Chemical Preservation of Perishable Products

  • Nitrate & Nitrite in Food Preservation
    03:15
  • Organic Acids Used in Food Preservation
    02:11
  • Chemical Preservation of Perishable Products
    01:16

ATP

  • Energy Coupling
    04:57
  • ATP
    06:44
  • Phosphorylation
    03:00

Enzymes

  • Functions of Enzymes
    02:32
  • Enzymes Example 1
    02:25
  • Enzymes
    02:55

Enzyme Activation Energy

  • Enzyme Activation Energy
    04:18
  • Enzymes Lower EA
    03:26

Enzyme Binding Factors

  • Cofactors
    04:22
  • Enzyme-Substrate Complex
    03:49

Enzyme Inhibition

  • Enzyme Inhibition
    05:03

Introduction to Metabolism

  • Introduction to Metabolism
    02:17
  • Catabolic & Anabolic Pathways
    03:25

Redox Reactions

  • Redox Reactions Example 1
    01:44
  • Electron Carriers: NADH & FADH2
    05:22
  • Redox Reactions
    04:46

Types of Phosphorylation

  • Substrate-Level Phosphorylation
    03:00
  • Oxidative Phosphorylation
    06:27
  • Types of Phosphorylation
    01:06

Introduction to Aerobic Cellular Respiration

  • Stages of Aerobic Cellular Respiration
    02:32
  • Introduction to Aerobic Cellular Respiration
    03:38
  • Introduction to Aerobic Cellular Respiration Example 2
    01:53

Glycolysis

  • Phases of Glycolysis
    05:57
  • Glycolysis Example 1
    01:29
  • Remembering Phases of Glycolysis
    02:49

Entner-Doudoroff Pathway

  • Entner-Doudoroff Pathway
    08:59

Pentose-Phosphate Pathway

  • Pentose-Phosphate Pathway
    04:44

Pyruvate Oxidation

  • Pyruvate Oxidation
    04:38

Krebs Cycle

  • Krebs Cycle Example 1
    01:20
  • Krebs Cycle
    01:37
  • Phases of The Krebs Cycle
    09:54

Electron Transport Chain

  • Remembering the ETC
    04:43
  • Electron Transport Chain in Prokaryotes
    02:49
  • Electron Transport Chain
    07:41

Chemiosmosis

  • Chemiosmosis
    05:01

Review of Aerobic Cellular Respiration

  • Total Products for Each Stage of Aerobic Cellular Respiration
    07:25
  • Review of Aerobic Cellular Respiration
    07:50

Fermentation & Anaerobic Respiration

  • Lactic Acid Fermentation
    03:33
  • What Happens to Aerobic Organisms if There's No Oxygen?
    07:50
  • Alcohol Fermentation
    01:59