Question 1

Which of the following is the correct procedure for cleaning your workspace before and after a microbiology lab session?

Accepted Answer

Wipe the benchtop with Lysol and wash your hands.

Question 2

Which of the following statements about lab safety is FALSE?

Accepted Answer

It is acceptable to chew gum in the lab if you are wearing gloves.

Question 3

Which bacterial arrangement is characterized by spherical cells forming irregular clusters?

Accepted Answer

Staphylococci

Question 4

Why are there no tetrads or sarcinae arrangements among bacilli?

Accepted Answer

Bacilli divide in only one plane.

Question 5

Which of the following best describes the shape and arrangement of Escherichia coli?

Accepted Answer

Individual bacilli

Question 6

A student observes a thin, flexible spiral bacterium under the microscope. Which type of spiral bacteria is this most likely to be?

Accepted Answer

Spirochete

Question 7

Which of the following is a eukaryotic microorganism commonly observed in the lab and reproduces by budding?

Accepted Answer

Saccharomyces cerevisiae

Question 8

When using oil immersion microscopy (1000x), the distance between two lines on the ocular micrometer represents approximately:

Accepted Answer

$$1\ \mu m$$

Question 9

A student measures a yeast cell under oil immersion and finds it spans 4 divisions on the ocular micrometer. What is the diameter of the yeast cell?

Accepted Answer

$$4\ \mu m$$

Question 10

Which of the following best explains why some bacterial arrangements appear as clusters or are broken apart when observed under the microscope?

Accepted Answer

Slide preparation can cause clumping or separation of cells.

Question 11

Which of the following is NOT a correct pairing of bacterial genus and arrangement?

Accepted Answer

Streptococcus – clusters

Question 12

Which of the following is the correct order of steps for preparing a wet mount of yeast?

Accepted Answer

Place a drop of yeast culture on a slide, cover with a coverslip, observe under oil immersion.

Question 13

Which of the following best describes the arrangement of Bacillus megaterium as seen under the microscope?

Accepted Answer

Streptobacilli

Question 14

A student is late to lab and uses their cell phone during the session. According to lab policy, what is the consequence?

Accepted Answer

They will lose a portion of their attendance points for that day.

Question 15

Which of the following is the best method to improve contrast when observing a wet mount of yeast under the microscope?

Accepted Answer

Reduce the light using the iris diaphragm lever.

Question 16

Which of the following is a correct statement about the measurement of microorganisms using the ocular micrometer?

Accepted Answer

The calibration is only accurate at the magnification used for calibration.

Question 17

Which of the following is the most likely reason for observing cocci in pairs (diplococci) under the microscope?

Accepted Answer

The bacteria divide in one plane and remain attached after division.

Question 18

Which of the following is the correct disposal method for a coverslip after preparing a wet mount?

Accepted Answer

Place it in the broken glass disposal container.

Question 19

Which of the following best describes the appearance of spirochetes under the microscope?

Accepted Answer

Extremely thin, wavy lines

Question 20

A student observes a field of bacteria under oil immersion and counts 10 divisions of the ocular micrometer spanning a single bacterium. What is the length of the bacterium?

Accepted Answer

$$10\ \mu m$$

Microbiology Lab 1 Study Guide

Guida di studio - Domande di pratica

Study guide - Flashcard