$$TM = \text{ocular lens power} \times \text{objective lens power}$$

$$\text{Diameter B} = \frac{4 \text{ mm} \times 40x}{400x} = 0.4 \text{ mm}$$

Question 1

When using a compound microscope, what is the correct formula to calculate the total magnification (TM) of a specimen?

Accepted Answer

$$TM = 	ext{ocular lens power} 	imes 	ext{objective lens power}$$

Question 2

A microscope has an ocular lens of 10x and an objective lens of 40x. What is the total magnification?

Accepted Answer

$$10x 	imes 40x = 400x$$

Question 3

If the diameter of field A is 4 mm at 40x magnification, what is the diameter of field B at 400x magnification?

Accepted Answer

$$	ext{Diameter B} = \frac{4 	ext{ mm} 	imes 40x}{400x} = 0.4 	ext{ mm}$$

Question 4

Which part of the compound microscope should you use to make large adjustments to the focus when first viewing a specimen?

Accepted Answer

Coarse adjustment knob

Question 5

Why should you only use the fine adjustment knob when focusing with the 40x objective lens?

Accepted Answer

The working distance is very small and using the coarse knob may break the slide.

Question 6

Which organelle is responsible for synthesizing proteins in the cell?

Accepted Answer

Ribosome

Question 7

During which stage of mitosis do chromosomes align at the center of the cell?

Accepted Answer

Metaphase

Question 8

A student observes three colored threads on a slide under a microscope. Which concept is being demonstrated?

Accepted Answer

Depth of field

Question 9

Which organelle is the site of ATP production in eukaryotic cells?

Accepted Answer

Mitochondrion

Question 10

What is the function of the Golgi apparatus in the cell?

Accepted Answer

Modifies and exports proteins

Question 11

Which structure organizes the spindle during cell division?

Accepted Answer

Centrosome

Question 12

A cell is observed with its nuclear envelope reforming and chromosomes at opposite ends. Which stage of mitosis is this?

Accepted Answer

Telophase

Question 13

Which cell structure controls passage of molecules in and out of the cell?

Accepted Answer

Cell membrane

Question 14

If you want to clean the lenses of a microscope, which material should you use?

Accepted Answer

Special grit-free lens paper

Question 15

Which organelle digests food vacuoles and damaged organelles?

Accepted Answer

Lysosome

Question 16

What is the main function of microtubules in the cell?

Accepted Answer

Allows movement of organelles within the cell

Question 17

Which of the following best describes the process of cytokinesis?

Accepted Answer

Cytoplasmic division resulting in two daughter cells

Question 18

A student is using a microscope and switches from a 10x objective to a 40x objective. What happens to the field of view diameter?

Accepted Answer

It decreases fourfold

Question 19

Which cell structure is responsible for shuttling proteins between organelles?

Accepted Answer

Transport vesicles

Question 20

Which of the following is a correct safety procedure when handling a compound microscope?

Accepted Answer

Carry it with two hands, supporting the base and arm

Microscopy and Cellular Anatomy: Study Notes for Anatomy & Physiology

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