how-does-2d-electrophoresis-separate-proteins-with-identical-molecular-weights-b

Question

How does 2D electrophoresis separate proteins with identical molecular weights but different isoelectric points?

Accepted Answer

By using isoelectric focusing to separate based on isoelectric points first, followed by SDS PAGE.

Answer

By using a combination of hydrophobic interaction chromatography and SDS PAGE.

Answer

By using SDS PAGE to separate based on molecular weights first, followed by isoelectric focusing.

Answer

By using affinity chromatography followed by isoelectric focusing.