what-is-the-rationale-for-starting-with-the-longest-fragment-when-ordering-pepti

Question

What is the rationale for starting with the longest fragment when ordering peptide fragments?

Accepted Answer

The longest fragment provides the most information for aligning other fragments.

Answer

The longest fragment contains the most hydrophobic residues.

Answer

The longest fragment is always the N-terminal fragment.

Answer

The longest fragment is less likely to be degraded during sequencing.