how-does-the-rate-limiting-step-of-an-enzyme-catalyzed-reaction-affect-kcat-and-

Question

How does the rate-limiting step of an enzyme-catalyzed reaction affect kcat and the overall reaction rate?

Accepted Answer

The rate-limiting step determines kcat, which in turn dictates the maximum possible reaction rate.

Answer

The rate-limiting step increases the overall reaction rate by increasing kcat.

Answer

The rate-limiting step only affects the binding affinity of the enzyme.

Answer

The rate-limiting step has no effect on kcat or the overall reaction rate.