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1. Introduction to Biochemistry

2. Water

3. Amino Acids

4. Protein Structure

5. Protein Techniques

6. Enzymes and Enzyme Kinetics

7. Enzyme Inhibition and Regulation 

8. Protein Function 

9. Carbohydrates

10. Lipids

11. Biological Membranes and Transport 

12. Biosignaling

Review 1: Nucleic Acids, Lipids, & Membranes

Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway

Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism

Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation

Which of the following is a limitation of using anion-exchange chromatography for protein purification?