which-component-of-pyrosequencing-is-directly-responsible-for-detecting-nucleoti

Question

Which component of pyrosequencing is directly responsible for detecting nucleotide incorporation?

Accepted Answer

Pyrophosphate release triggers a light signal via luciferase.

Answer

Fluorescent tags are used to label incorporated nucleotides.

Answer

DNA polymerase activity is measured directly.

Answer

Radioactive decay is monitored for nucleotide incorporation.