why-are-basic-conditions-ph-9-important-for-the-first-reaction-in-edman-degradat

Question

Why are basic conditions (pH ~9) important for the first reaction in Edman degradation?

Accepted Answer

They facilitate the reaction of phenyl isothiocyanate with the N-terminal amino acid.

Answer

They are necessary for the action of trifluoroacetic acid.

Answer

They help in cleaving the peptide chain.

Answer

They stabilize the PTH amino acid for identification.