in-an-hplc-chromatogram-how-can-you-distinguish-between-peaks-corresponding-to-d

Question

In an HPLC chromatogram, how can you distinguish between peaks corresponding to DNP derivatives and free amino acids?

Accepted Answer

DNP derivatives have distinct retention times compared to free amino acids.

Answer

DNP derivatives are not visible in the chromatogram.

Answer

DNP derivatives elute before free amino acids.

Answer

DNP derivatives have higher absorbance than free amino acids.