why-is-salt-added-to-a-cation-exchange-chromatography-column-to-elute-positively

Question

Why is salt added to a cation exchange chromatography column to elute positively charged proteins?

Accepted Answer

Salt decreases the ionic interactions between proteins and the resin.

Answer

Salt enhances the charge of proteins, making them more mobile.

Answer

Salt neutralizes the charge of the resin.

Answer

Salt increases the binding strength of proteins to the resin.