a-researcher-is-using-edman-degradation-to-sequence-a-peptide-what-is-the-sequen

Question

A researcher is using Edman degradation to sequence a peptide. What is the sequence of steps they should follow?

Accepted Answer

Sequentially remove and identify the N-terminal amino acid, repeating the process for each subsequent residue.

Answer

Use chemical cleavage to break the peptide into fragments, then sequence each fragment.

Answer

Hydrolyze the peptide into free amino acids and determine their composition.

Answer

Simultaneously remove all amino acids and identify them using mass spectrometry.