how-does-sds-page-separate-protein-subunits-without-cleaving-disulfide-bonds

Question

How does SDS-PAGE separate protein subunits without cleaving disulfide bonds?

Accepted Answer

By denaturing the quaternary structure but leaving disulfide bonds intact

Answer

By cleaving all covalent bonds including disulfide bonds

Answer

By reducing the protein to its primary structure

Answer

By breaking hydrogen bonds and ionic interactions only