given-a-series-of-purification-steps-how-would-you-design-an-experiment-to-deter

Question

Given a series of purification steps, how would you design an experiment to determine which step most effectively increases specific activity?

Accepted Answer

Calculate specific activity at each step and compare the increases.

Answer

Measure the total protein content at each step.

Answer

Determine the molecular weight of proteins at each step.

Answer

Assess the color change of the protein solution at each step.