in-a-laboratory-setting-why-might-studying-an-enzyme-at-saturating-substrate-con

Question

In a laboratory setting, why might studying an enzyme at saturating substrate concentrations not provide an accurate measure of its binding affinity?

Accepted Answer

Because all enzyme molecules are bound to substrate, masking differences in binding affinity.

Answer

Because enzyme-substrate complexes do not form at high concentrations.

Answer

Because enzyme activity is too low to measure accurately.

Answer

Because substrate concentration is irrelevant to enzyme function.