why-is-the-steady-state-assumption-crucial-for-deriving-the-michaelis-constant-k

Question

Why is the steady state assumption crucial for deriving the Michaelis constant (Km) and the Michaelis-Menten equation?

Accepted Answer

It allows the simplification of complex kinetic equations by assuming constant enzyme-substrate complex concentration.

Answer

It assumes that the substrate concentration is always higher than the enzyme concentration.

Answer

It assumes that the enzyme concentration is always higher than the substrate concentration.

Answer

It assumes that the product concentration is negligible.