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Which of the following correctly arranges the major steps of eukaryotic gene expression in chronological order?
A
Translation → Transcription → RNA processing → Protein modification
B
Transcription → Translation → RNA processing → Protein modification
C
RNA processing → Transcription → Translation → Protein modification
D
Transcription → RNA processing → Translation → Protein modification
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1
Understand the process of eukaryotic gene expression, which involves multiple steps to convert genetic information in DNA into a functional protein.
Step 1: Transcription occurs first. During this step, the DNA sequence of a gene is transcribed into messenger RNA (mRNA). This process takes place in the nucleus of eukaryotic cells.
Step 2: RNA processing follows transcription. The pre-mRNA undergoes modifications such as the addition of a 5' cap, splicing to remove introns, and the addition of a poly-A tail at the 3' end. This step ensures the mRNA is mature and ready for translation.
Step 3: Translation is the next step. The mature mRNA is transported out of the nucleus to the ribosome, where it is translated into a polypeptide chain (protein) using the genetic code.
Step 4: Protein modification occurs after translation. The newly synthesized protein may undergo post-translational modifications, such as folding, cleavage, or the addition of functional groups, to become fully functional.