what-structural-difference-between-ddntps-and-normal-nucleotides-causes-terminat

Question

What structural difference between ddNTPs and normal nucleotides causes termination of DNA synthesis in Sanger sequencing?

Accepted Answer

Lack of a 3' hydroxyl group in ddNTPs.

Answer

Lack of a 5' phosphate group in ddNTPs.

Answer

Presence of a methyl group in ddNTPs.

Answer

Presence of an extra phosphate group in ddNTPs.