Ch. 12 - The Genetic Code and Transcription

Klug - Essentials of Genetics 10th Edition

Klug10th EditionEssentials of GeneticsISBN: 9780135588789Non è quello che usi tu?Cambia libro di testo

Klug 10th Edition

Ch. 12 - The Genetic Code and Transcription

Problema 23e

Capitolo 12, Problema 23e

Shown here are the amino acid sequences of the wild-type and three mutant forms of a short protein.
_
Wild-type: Met-Trp-Tyr-Arg-Gly-Ser-Pro-Thr
Mutant 1: Met-Trp
Mutant 2: Met-Trp-His-Arg-Gly-Ser-Pro-Thr
Mutant 3: Met -Cys-Ile-Val-Val-Val-Gln-His
_
Use this information to answer the following questions:
Another mutation (mutant 4) is isolated. Its amino acid sequence is unchanged from the wild type, but the mutant cells produce abnormally low amounts of the wild-type proteins. As specifically as you can, predict where this mutation exists in the gene.

Guida verificata passo dopo passo

Understand that the amino acid sequence of mutant 4 is identical to the wild-type protein, indicating that the mutation does not change the protein's primary structure.

Since the protein sequence is unchanged but the amount of protein produced is abnormally low, consider mutations that affect gene expression rather than protein coding, such as mutations in regulatory regions.

Identify key regulatory regions in the gene that control transcription and translation, including the promoter, enhancer, and possibly the 5' untranslated region (5' UTR) or sequences affecting mRNA stability.

Predict that the mutation likely exists in the promoter or other regulatory elements upstream of the coding sequence, which would reduce transcription efficiency and thus lower protein production without altering the amino acid sequence.

Conclude that the mutation is not in the coding region but in a regulatory region of the gene that controls the amount of protein synthesized.

Risposta video verificata per un problema simile:

Questa soluzione video è stata consigliata dai nostri tutor come utile per risolvere questo problema.

Durata del video:

Concetti chiave

Ecco i concetti essenziali che devi comprendere per rispondere correttamente alla domanda.

Gene Expression and Protein Synthesis

Gene expression involves transcribing DNA into mRNA and translating mRNA into a protein sequence. Mutations can affect protein quantity or quality by altering transcription, mRNA stability, or translation efficiency, even if the amino acid sequence remains unchanged.

Video consigliato:

Percorso guidato

05:05

Proteins

Types of Mutations Affecting Protein Levels

Mutations outside the coding region, such as in promoters, enhancers, or regulatory sequences, can reduce protein production without changing the amino acid sequence. These regulatory mutations impact transcription initiation or mRNA processing, leading to lower protein amounts.

Video consigliato:

Percorso guidato

10:48

Mutations and Phenotypes

Relationship Between DNA Sequence and Protein Sequence

The amino acid sequence is determined by the coding DNA sequence, but mutations in non-coding regions or synonymous codons can affect gene expression levels. Thus, unchanged protein sequences can still result from mutations that alter gene regulation rather than protein structure.

Video consigliato:

Percorso guidato

08:55

Sequencing Overview

Pratica correlata

Domanda del libro di testo

Shown here are the amino acid sequences of the wild-type and three mutant forms of a short protein.

___________________________________________________

Wild-type: Met-Trp-Tyr-Arg-Gly-Ser-Pro-Thr

Mutant 1: Met-Trp

Mutant 2: Met-Trp-His-Arg-Gly-Ser-Pro-Thr

Mutant 3: Met-Cys-Ile-Val-Val-Val-Gln-Hi

___________________________________________________

Use this information to answer the following questions:

For each mutant protein, determine the specific ribonucleotide change that led to its synthesis.

479

views

Domanda del libro di testo

Recent observations indicate that alternative splicing is a common way for eukaryotes to expand their repertoire of gene functions. Studies indicate that approximately 50 percent of human genes exhibit alternative splicing and approximately 15 percent of disease-causing mutations involve aberrant alternative splicing. Different tissues show remarkably different frequencies of alternative splicing, with the brain accounting for approximately 18 percent of such events [Xu et al. (2002). Nucl. Acids Res. 30:3754–3766].

Why might some tissues engage in more alternative splicing than others?

441

views

Domanda del libro di testo

Shown here are the amino acid sequences of the wild-type and three mutant forms of a short protein.

__________________________________________________

Wild-type: Met-Trp-Tyr-Arg-Gly-Ser-Pro-Thr

Mutant 1: Met-Trp

Mutant 2: Met-Trp-His-Arg-Gly-Ser-Pro-Thr

Mutant 3: Met -Cys-Ile-Val-Val-Val-Gln-His

______________________________________________

Use this information to answer the following questions:

The wild-type RNA consists of nine triplets. What is the role of the ninth triplet?

500

views

Domanda del libro di testo

Shown here are the amino acid sequences of the wild-type and three mutant forms of a short protein.

___________________________________________________

Wild-type: Met-Trp-Tyr-Arg-Gly-Ser-Pro-Thr

Mutant 1: Met-Trp

Mutant 2: Met-Trp-His-Arg-Gly-Ser-Pro-Thr

Mutant 3: Met-Cys-Ile-Val-Val-Val-Gln-Hi

___________________________________________________

Use this information to answer the following questions:

Of the first eight wild-type triplets, which, if any, can you determine specifically from an analysis of the mutant proteins? In each case, explain why or why not.

566

views