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Ch. 8 - Genetic Analysis and Mapping in Bacteria and Bacteriophages
Klug - Essentials of Genetics 10th Edition
Klug10th EditionEssentials of GeneticsISBN: 9780135588789Non è quello che usi tu?Cambia libro di testo
Capitolo 8, Problema 10

The bacteriophage genome consists of many genes encoding proteins that make up the head, collar, tail, and tail fibers. When these genes are transcribed following phage infection, how are these proteins synthesized, since the phage genome lacks genes essential to ribosome structure?

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1
Understand that bacteriophages are viruses that infect bacteria and rely on the host's cellular machinery to reproduce.
Recognize that although the phage genome encodes proteins for its structure (head, collar, tail, tail fibers), it does not encode ribosomal RNA or ribosomal proteins, which are essential components of ribosomes.
Recall that ribosomes are the molecular machines responsible for translating mRNA into proteins, and these are provided by the bacterial host cell.
Conclude that after the phage injects its genome into the bacterial host, the host's ribosomes translate the phage mRNAs into phage proteins.
Therefore, the synthesis of phage structural proteins depends on the host bacterium's ribosomes and translation machinery, which the phage genome does not encode.

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Bacteriophage Genome and Gene Encoding

Bacteriophages have genomes encoding structural proteins like head, collar, tail, and tail fibers. These genes provide instructions for making viral components but do not include genes for the host's cellular machinery, such as ribosomes.
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Percorso guidato
06:04
Mapping Bacteriophages

Host Machinery Utilization by Viruses

Viruses, including bacteriophages, rely on the host cell's molecular machinery to replicate and produce proteins. Since phages lack ribosomal genes, they hijack the host bacterium's ribosomes and enzymes to translate their mRNA into proteins.
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Percorso guidato
03:44
Plaques and Experiments

Transcription and Translation in Phage Infection

After infection, phage DNA is transcribed into mRNA using host RNA polymerase. This mRNA is then translated by host ribosomes into viral proteins, enabling the assembly of new phage particles within the bacterial cell.
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Percorso guidato
07:58
Translation initiation