Domanda del libro di testo
Define and compare the four levels of protein organization.
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Ch. 14 - Translation and Proteins
Klug12th EditionConcepts of GeneticsISBN: 9780135564776
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TOC.tableOfContents
- Ch. 1 - Introduction to Genetics17
- Ch. 2 - Mitosis and Meiosis36
- Ch. 3 - Mendelian Genetics51
- Ch. 4 - Extensions of Mendelian Genetics74
- Ch. 5 - Chromosome Mapping in Eukaryotes51
- Ch. 6 - Genetic Analysis and Mapping in Bacteria and Bacteriophages46
- Ch. 7 - Sex Determination and Sex Chromosomes33
- Ch. 8 - Chromosome Mutations: Variation in Number and Arrangement40
- Ch. 9 - Extranuclear Inheritance31
- Ch. 10 - DNA Structure and Analysis43
- Ch. 11 - DNA Replication and Recombination44
- Ch. 12 - DNA Organization in Chromosomes30
- Ch. 13 - The Genetic Code and Transcription54
- Ch. 14 - Translation and Proteins47
- Ch. 15 - Gene Mutation, DNA Repair, and Transposition41
- Ch. 16 - Regulation of Gene Expression in Bacteria29
- Ch. 17 - Transcriptional Regulation in Eukaryotes41
- Ch. 18 - Post-transcriptional Regulation in Eukaryotes33
- Ch. 19 - Epigenetics33
- Ch. 20 - Recombinant DNA Technology44
- Ch. 21 - Genomic Analysis36
- Ch. 22 - Applications of Genetic Engineering and Biotechnology36
- Ch. 23 - Developmental Genetics37
- Ch. 24 - Cancer Genetics34
- Ch. 25 - Quantitative Genetics and Multifactorial Traits54
- Ch. 26 - Population and Evolutionary Genetics45
Klug12th EditionConcepts of GeneticsISBN: 9780135564776Non è quello che usi tu?Cambia libro di testo
Capitolo 14, Problema 21
Hemoglobins from two individuals are compared by electrophoresis and by fingerprinting. Electrophoresis reveals no difference in migration, but fingerprinting shows an amino acid difference. How is this possible?
Guida verificata passo dopo passo1
Understand that electrophoresis separates proteins based on their charge and size. If two hemoglobins migrate the same, they likely have similar overall charge and size.
Recognize that fingerprinting involves breaking down the protein into smaller peptide fragments and analyzing these fragments, often by chromatography or mass spectrometry.
Consider that a single amino acid change might not significantly alter the overall charge or size of the hemoglobin protein, thus not affecting its migration in electrophoresis.
Realize that fingerprinting is more sensitive to small changes, such as a single amino acid substitution, because it analyzes the specific sequence of peptides.
Conclude that the amino acid difference detected by fingerprinting could be a substitution that does not affect the charge or size of the protein enough to alter its electrophoretic mobility.
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Electrophoresis
Electrophoresis is a laboratory technique used to separate macromolecules, such as proteins or nucleic acids, based on their size and charge. In the context of hemoglobins, this method allows for the visualization of protein variants by their migration through a gel under an electric field. If two hemoglobins migrate identically, it suggests that they have similar charge and size, indicating no detectable differences in their overall structure.
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06:36
Proteomics
Amino Acid Substitution
Amino acid substitution refers to a change in the protein sequence where one amino acid is replaced by another. This can occur due to mutations in the DNA that codes for the protein. While such substitutions may not affect the overall charge or size of the protein significantly enough to alter its migration in electrophoresis, they can still be detected through more sensitive methods like fingerprinting, which analyzes the specific sequence of amino acids.
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Protein Fingerprinting
Protein fingerprinting is a technique used to identify proteins based on their unique patterns of amino acids or peptide fragments. This method can reveal subtle differences between proteins that may not be apparent through electrophoresis. Fingerprinting can detect variations in amino acid composition or sequence, allowing for the identification of distinct protein variants even when they exhibit similar physical properties in other analyses.
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05:05Proteins
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