What would be the likely impact on the rate of a biochemical reaction catalyzed by an enzyme that functions optimally at room temperature (about 25°C) if a reducing agent like dithiothreitol (DTT) is introduced?

The rate of the biochemical reaction will increase significantly.

The rate of the biochemical reaction will decrease significantly.

There will be no change in the rate of the biochemical reaction.

The effect on the rate of the biochemical reaction cannot be determined.