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Ch. 9 - Controlling Microbial Growth in the Environment
Bauman - Microbiology with Diseases by Taxonomy 6th Edition
Bauman6th EditionMicrobiology with Diseases by TaxonomyISBN: 9780134832302Non è quello che usi tu?Cambia libro di testo
Capitolo 9, Problema 6

The endospores of which organism can be used as a biological indicator of sterilization?
a. Bacillus stearothermophilus
b. Salmonella enterica
c. Mycobacterium tuberculosis
d. Staphylococcus aureus

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1
Understand that biological indicators for sterilization are typically highly resistant bacterial endospores used to test the effectiveness of sterilization processes.
Recall that Bacillus species are commonly used as biological indicators because their endospores are very resistant to heat and chemical sterilization methods.
Identify that Bacillus stearothermophilus is a thermophilic bacterium whose endospores are especially resistant to moist heat sterilization (autoclaving), making it a standard biological indicator for steam sterilization.
Recognize that the other organisms listed (Salmonella enterica, Mycobacterium tuberculosis, Staphylococcus aureus) do not form endospores and therefore are not suitable as biological indicators for sterilization.
Conclude that the correct organism whose endospores are used as a biological indicator of sterilization is Bacillus stearothermophilus.

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Endospores and Their Resistance

Endospores are highly resistant, dormant structures formed by certain bacteria to survive extreme conditions. Their resistance to heat, chemicals, and radiation makes them ideal for testing sterilization processes, as surviving endospores indicate ineffective sterilization.
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Biological Indicators in Sterilization

Biological indicators use resistant microorganisms, like bacterial endospores, to verify sterilization efficacy. If the indicator spores are killed during sterilization, it confirms the process was successful; if not, sterilization failed.
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Bacillus stearothermophilus as a Sterilization Indicator

Bacillus stearothermophilus produces heat-resistant endospores commonly used as biological indicators for steam sterilization (autoclaving). Their high resistance to moist heat makes them a standard choice to validate sterilization cycles.
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