Domanda del libro di testo
Use the following choices to answer questions 4 and 5:
a. Anti-Brucella
b. Brucella
c. Substrate for the enzyme
Which item is from the patient in an indirect ELISA test?
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Ch. 18 - Practical Applications of Immunology
Tortora14th EditionMicrobiology: An IntroductionISBN: 9780138200398
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TOC.tableOfContents
- Ch. 1 - The Microbial World and You18
- Ch. 2 - Chemical Principles20
- Ch. 3 - Observing Microorganisms Through a Microscope18
- Ch. 7 - The Control of Microbial Growth20
- Ch. 9 - Biotechnology & DNA Technology20
- Ch. 10 - Classification of Microorganisms15
- Ch. 11 - The Prokaryotes: Domains Bacteria and Archaea14
- Ch. 12 - The Eukaryotes: Fungi, Algae, Protozoa, and Helminths17
- Ch. 13 - Viruses, Viroids, and Prions20
- Ch. 14 - Principles of Disease and Epidemiology21
- Ch. 15 - Microbial Mechanisms of Pathogenicity19
- Ch. 16 - Innate Immunity: Nonspecific Defenses of the Host20
- Ch. 17 - Adaptive Immunity: Specific Defenses of the Host20
- Ch. 18 - Practical Applications of Immunology20
- Ch. 19 - Disorders Associated with the Immune System19
- Ch. 20 - Antimicrobial Drugs19
- Ch. 21 - Microbial Diseases of the Skin and Eyes21
- Ch. 22 - Microbial Diseases of the Nervous System19
- Ch. 23 - Microbial Diseases of the Cardiovascular and Lymphatic Systems20
- Ch. 24 - Microbial Diseases of the Respiratory System20
- Ch. 25 - Microbial Diseases of the Digestive System20
- Ch. 26 - Microbial Diseases of the Urinary and Reproductive Systems19
- Ch. 27 - Environmental Microbiology20
- Ch. 28 - Applied and Industrial Microbiology19
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Capitolo 14, Problema 4
How are monoclonal antibodies produced?
Guida verificata passo dopo passo1
Understand that monoclonal antibodies are identical antibodies produced by a single clone of B cells, which means they are specific to one epitope of an antigen.
Begin by immunizing a suitable animal, commonly a mouse, with the antigen of interest to stimulate the production of B cells that generate antibodies against that antigen.
Harvest the spleen cells from the immunized animal, as these contain the antibody-producing B cells.
Fuse these spleen B cells with immortal myeloma (cancer) cells to create hybridoma cells; this fusion combines the antibody-producing ability of B cells with the longevity and growth properties of myeloma cells.
Select and culture the hybridoma cells in a selective medium (such as HAT medium) that allows only fused hybrid cells to survive, then screen these hybridomas to identify and isolate those producing the desired monoclonal antibody.
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Hybridoma Technology
Hybridoma technology is the process used to produce monoclonal antibodies by fusing a specific antibody-producing B cell with a myeloma (cancer) cell. This fusion creates a hybrid cell line that can both produce a single type of antibody and proliferate indefinitely in culture.
Video consigliato:
02:30
Introduction to DNA-Based Technology
Monoclonal Antibodies
Monoclonal antibodies are identical antibodies produced by a single clone of B cells, targeting one specific epitope on an antigen. They are highly specific and uniform, making them valuable for diagnostics, therapeutics, and research.
Video consigliato:
02:34Antibody Diversity
Selection and Screening of Hybridomas
After fusion, hybridoma cells are cultured in selective media to eliminate unfused cells. The surviving hybridomas are screened to identify those producing the desired antibody, which are then cloned to ensure monoclonality and expanded for antibody production.
Video consigliato:
03:27Selective Media
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