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What is the purpose of a counterstain in the acid-fast stain?
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Ch. 3 - Observing Microorganisms Through a Microscope
Tortora14th EditionMicrobiology: An IntroductionISBN: 9780138200398
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TOC.tableOfContents
- Ch. 1 - The Microbial World and You18
- Ch. 2 - Chemical Principles20
- Ch. 3 - Observing Microorganisms Through a Microscope18
- Ch. 7 - The Control of Microbial Growth20
- Ch. 9 - Biotechnology & DNA Technology20
- Ch. 10 - Classification of Microorganisms15
- Ch. 11 - The Prokaryotes: Domains Bacteria and Archaea14
- Ch. 12 - The Eukaryotes: Fungi, Algae, Protozoa, and Helminths17
- Ch. 13 - Viruses, Viroids, and Prions20
- Ch. 14 - Principles of Disease and Epidemiology21
- Ch. 15 - Microbial Mechanisms of Pathogenicity19
- Ch. 16 - Innate Immunity: Nonspecific Defenses of the Host20
- Ch. 17 - Adaptive Immunity: Specific Defenses of the Host20
- Ch. 18 - Practical Applications of Immunology20
- Ch. 19 - Disorders Associated with the Immune System19
- Ch. 20 - Antimicrobial Drugs19
- Ch. 21 - Microbial Diseases of the Skin and Eyes21
- Ch. 22 - Microbial Diseases of the Nervous System19
- Ch. 23 - Microbial Diseases of the Cardiovascular and Lymphatic Systems20
- Ch. 24 - Microbial Diseases of the Respiratory System20
- Ch. 25 - Microbial Diseases of the Digestive System20
- Ch. 26 - Microbial Diseases of the Urinary and Reproductive Systems19
- Ch. 27 - Environmental Microbiology20
- Ch. 28 - Applied and Industrial Microbiology19
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Tortora 14th EditionCh. 3 - Observing Microorganisms Through a MicroscopeProblema 6
Tortora 14th EditionCh. 3 - Observing Microorganisms Through a MicroscopeProblema 6Capitolo 3, Problema 6
Which of the following pairs is mismatched?
a. Capsule—negative stain
b. Cell arrangement—simple stain
c. Cell size—negative stain
d. Gram stain—bacterial identification
e. None of the above
Guida verificata passo dopo passo1
Step 1: Understand the purpose of each staining technique mentioned in the options. For example, a negative stain is used to visualize capsules and cell size by staining the background, leaving the cells or capsules clear.
Step 2: Analyze option (a): 'capsule—negative stain'. Since negative staining highlights capsules by staining the background and leaving the capsule unstained, this pair is correctly matched.
Step 3: Analyze option (b): 'cell arrangement—simple stain'. Simple stains color the cells uniformly, which helps in observing cell shape and arrangement, so this pair is also correctly matched.
Step 4: Analyze option (c): 'cell size—negative stain'. Negative staining is indeed used to observe cell size because it does not shrink or distort cells, so this pair is correctly matched as well.
Step 5: Analyze option (d): 'Gram stain—bacterial identification'. Gram staining differentiates bacteria into Gram-positive and Gram-negative groups, which is a fundamental step in bacterial identification, so this pair is correctly matched. Therefore, if all pairs are correctly matched, the answer would be (e) none of the above.
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Negative Stain Technique
Negative staining uses acidic dyes that stain the background but not the cells, highlighting structures like capsules and cell size without heat-fixing. It is useful for observing delicate structures and true cell morphology.
Video consigliato:
02:10
Introduction to Staining
Simple Stain and Cell Arrangement
Simple staining employs a single basic dye to color cells, enhancing contrast to observe cell shape and arrangement. It helps identify how bacterial cells group together, such as chains or clusters.
Video consigliato:
03:23Simple Staining
Gram Stain and Bacterial Identification
The Gram stain differentiates bacteria into Gram-positive or Gram-negative based on cell wall properties. This differential staining is fundamental for bacterial classification and guides identification and treatment.
Video consigliato:
04:57Gram Stain
Pratica correlata
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Why is a mordant used in the Gram stain? In the flagella stain?
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Domanda del libro di testo
The maximum magnification of a compound microscope is (a) ________; that of an electron microscope, (b) ________. The maximum resolution of a compound microscope is (c) ________; that of an electron microscope, (d) ________. One advantage of a scanning electron microscope over a transmission electron microscope is (e) ________.
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Domanda del libro di testo
Assume that you are viewing a Gram-stained field of red cocci and blue rods through the microscope. You can safely conclude that you have
a. Made a mistake in staining
b. Two different species
c. Old bacterial cells
d. Young bacterial cells
e. None of the above
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Domanda del libro di testo
Which of the following is not a functionally analogous pair of stains?
a. Nigrosin and malachite green
b. Crystal violet and carbolfuchsin
c. Safranin and methylene blue
d. Ethanol-acetone and acid-alcohol
e. All of the above pairs are functionally analogous.
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Domanda del libro di testo
Assume you stain Clostridium by applying a basic stain, carbolfuchsin, with heat, decolorizing with acid-alcohol, and counterstaining with an acidic stain, nigrosin. Through the microscope, the endospores are 1, and the cells are stained 2.
a. 1—red; 2—black
b. 1—black; 2—colorless
c. 1—colorless; 2—black
d. 1—red; 2—colorless
e. 1—black; 2—red
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