explain-why-cysteine-pi-5-15-elutes-before-isoleucine-pi-5-60-when-their-solutio

Question

Explain why cysteine (pI = 5.15) elutes before isoleucine (pI = 5.60) when their solution having pH 5 is passed through a cation-exchange column.

Accepted Answer

Because cysteine has a higher amount of negative charge at this pH due to its lower pI value

Answer

Because cysteine has a higher amount of positive charge at this pH due to its lower pI value

Answer

Because isoleucine has a higher amount of negative charge at this pH due to its higher pI value

Answer

Because isoleucine has a higher amount of positive charge at this pH due to its lower pI value