Q: If the diameter of the field of view at 4x magnification is 4 mm, what is the diameter at 40x magnification?

$$\frac{4 \text{ mm} \times 4x}{40x} = 0.4 \text{ mm}$$

Q: A student observes that the field of view decreases as magnification increases. Which formula explains this relationship?

$$\text{Field diameter at new magnification} = \frac{\text{Field diameter at old magnification} \times \text{Old magnification}}{\text{New magnification}}$$

Question 1

Which part of the compound microscope is responsible for focusing light onto the specimen?

Accepted Answer

Condenser

Question 2

A student uses a 10x ocular lens and a 40x objective lens. What is the total magnification achieved?

Accepted Answer

$$10 	imes 40 = 400$$

Question 3

Why is a compound microscope called 'compound'?

Accepted Answer

It uses two sets of lenses to achieve higher magnification.

Question 4

Which objective lens provides the longest working distance?

Accepted Answer

4x objective

Question 5

If the diameter of the field of view at 4x magnification is 4 mm, what is the diameter at 40x magnification?

Accepted Answer

$$\frac{4 	ext{ mm} 	imes 4x}{40x} = 0.4 	ext{ mm}$$

Question 6

Which adjustment knob should be used at high power to avoid damaging the slide?

Accepted Answer

Fine adjustment knob

Question 7

What is the main function of the ocular lens (eyepiece) in a compound microscope?

Accepted Answer

It magnifies the real image formed by the objective lens.

Question 8

Which of the following best describes 'resolution' in microscopy?

Accepted Answer

The minimum distance at which two points can be seen as distinct.

Question 9

A student is having trouble distinguishing two close points under the microscope. Which adjustment would most likely improve resolution?

Accepted Answer

Increasing light intensity and moving the condenser closer to the stage

Question 10

Which scenario best illustrates the application of working distance in microscopy?

Accepted Answer

Using the 4x objective lens to safely focus on a thick tissue section

Question 11

If a microscope has a 10x ocular lens and a 100x oil immersion objective lens, what is the total magnification?

Accepted Answer

$$10 	imes 100 = 1000$$

Question 12

Which part of the microscope allows you to move the slide horizontally and vertically?

Accepted Answer

Mechanical stage controls

Question 13

What is the correct sequence of the light path in a compound microscope?

Accepted Answer

Source → Condenser → Specimen → Objective lens → Ocular lens

Question 14

Why is oil immersion used with the 100x objective lens?

Accepted Answer

To increase resolution at very high magnification

Question 15

A student observes that the field of view decreases as magnification increases. Which formula explains this relationship?

Accepted Answer

$$	ext{Field diameter at new magnification} = \frac{	ext{Field diameter at old magnification} 	imes 	ext{Old magnification}}{	ext{New magnification}}$$

Question 16

Which lens should you use first when focusing on a new specimen slide?

Accepted Answer

4x scanning objective

Question 17

What is the main risk of using the coarse adjustment knob at high power?

Accepted Answer

Crushing the slide

Question 18

Which of the following is NOT a function of the rotating nosepiece?

Accepted Answer

Focuses light onto the specimen

Question 19

A researcher needs to distinguish two bacteria that are very close together. Which factor is most important?

Accepted Answer

Resolution

Question 20

Which of the following statements about objective lenses is correct?

Accepted Answer

The 100x objective lens requires oil immersion for optimal resolution.

LAB Compound Microscope

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