Question 1

Which part of a compound light microscope is responsible for concentrating light onto the specimen and should be positioned as close to the stage as possible for optimal illumination?

Accepted Answer

Condenser

Question 2

A student is using a microscope with a 10X ocular lens and a 40X objective lens. What is the total magnification achieved?

Accepted Answer

$$10 	imes 40 = 400$$

Question 3

When increasing the magnification by switching to a higher power objective lens, what happens to the field of vision?

Accepted Answer

It decreases

Question 4

Which microscope component is used to regulate the amount of light passing through the specimen?

Accepted Answer

Iris diaphragm

Question 5

A blood smear is prepared by placing a drop of blood on a slide, spreading it thinly, and covering it with a coverslip. Which specimen preparation method does this describe?

Accepted Answer

Smear or drop

Question 6

Which of the following best describes the function of the coarse adjustment knob on a microscope?

Accepted Answer

It brings the specimen into rough focus by adjusting the distance between the objective lens and the specimen.

Question 7

If a specimen is viewed under a 100X oil immersion objective lens with a 10X ocular lens, what is the total magnification?

Accepted Answer

$$10 	imes 100 = 1000$$

Question 8

Which of the following statements about resolution in microscopy is correct?

Accepted Answer

Resolution is the ability to distinguish two closely positioned objects as separate.

Question 9

A student observes that as they increase magnification, the working distance between the objective lens and the slide:

Accepted Answer

Decreases

Question 10

Which of the following specimen preparation methods is best for observing chromosomes released from nuclei?

Accepted Answer

Squash

Question 11

Which of the following is NOT a primary germ layer formed during embryonic development?

Accepted Answer

Epidermis

Question 12

Which tissue type is primarily responsible for covering body surfaces and lining cavities?

Accepted Answer

Epithelial tissue

Question 13

A section is made perpendicular to the long axis of a structure. What is this type of section called?

Accepted Answer

Cross section

Question 14

Which of the following best describes the function of stem cells in the early embryo?

Accepted Answer

They differentiate into all cell types of the organism.

Question 15

If the minimum distance at which two points can be distinguished is $$0.2 \ \mu m$$, what is the resolving power of the microscope?

Accepted Answer

$$0.2 \ \mu m$$

Question 16

Which of the following is a function of the mechanical stage on a microscope?

Accepted Answer

It holds and moves the slide for precise positioning.

Question 17

A student is viewing a slide under the microscope and moves the slide to the right. In which direction does the image appear to move when viewed through the ocular lens?

Accepted Answer

To the left

Question 18

Which of the following is TRUE regarding the organization of tissues in the human body?

Accepted Answer

Tissues are groups of similar cells that carry out related activities.

Question 19

Which part of the microscope should be used for fine focusing after the specimen is roughly in view?

Accepted Answer

Fine adjustment knob

Question 20

Which of the following best describes the function of the ocular lens in a compound microscope?

Accepted Answer

It is the lens you look through and typically has a magnification of 10X.

Microscopic Anatomy: Microscopy, Cells, and Histology of Epithelial Tissues

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