Q: A laboratory technician counts 100 WBCs on a blood smear and finds 60 neutrophils, 30 lymphocytes, and 10 monocytes. What is the percentage of lymphocytes?

$$\frac{30}{100} \times 100 = 30\%$$

Question 1

Which of the following best describes the primary focus of hematology?

Accepted Answer

The study of formed elements of the blood and blood-forming tissue.

Question 2

A well-made peripheral blood smear should have which of the following features?

Accepted Answer

A gradual transition from thick to thin with a feathered edge at the thin end.

Question 3

Which stain is commonly used for routine microscopic examination of blood smears and contains both methylene blue and eosin?

Accepted Answer

Wright stain

Question 4

During the preparation of a peripheral blood film, which factor does NOT affect the quality of the smear?

Accepted Answer

Type of microscope used for examination

Question 5

Which of the following is a clinical significance of erythrocyte morphological alterations observed in a blood smear?

Accepted Answer

They are associated with many diseases, especially anemia.

Question 6

A patient’s blood smear shows erythrocytes with increased central pallor and decreased staining intensity. What is the most likely description for these cells?

Accepted Answer

Hypochromic erythrocytes

Question 7

Which of the following best defines 'anisocytosis' as seen in a peripheral blood film?

Accepted Answer

Variation in the size of erythrocytes

Question 8

If a blood smear is prepared from blood collected in EDTA, what is the recommended time frame for preparing the smear to ensure accurate morphologic studies?

Accepted Answer

Within 2 hours

Question 9

Which objective lens is most appropriate for detailed examination of erythrocyte morphology and performing a leukocyte differential count?

Accepted Answer

Oil-immersion objective

Question 10

A laboratory technician counts 100 WBCs on a blood smear and finds 60 neutrophils, 30 lymphocytes, and 10 monocytes. What is the percentage of lymphocytes?

Accepted Answer

$$\frac{30}{100} 	imes 100 = 30\%$$

Question 11

Which of the following is NOT a reason for performing a microscopic examination of the peripheral blood film?

Accepted Answer

To measure blood pressure

Question 12

Leukocytosis is defined as:

Accepted Answer

A WBC count above normal

Question 13

Which of the following best describes the function of methylene blue in Wright stain?

Accepted Answer

It stains DNA and nuclei blue.

Question 14

A blood smear shows erythrocytes that are smaller than normal. What is the term for this alteration?

Accepted Answer

Microcytosis

Question 15

Which of the following is a qualitative aspect of hematology?

Accepted Answer

The appearance of each type of cell

Question 16

A patient presents with anemia. Which of the following best explains the condition?

Accepted Answer

A decrease in the oxygen-carrying capacity of the blood resulting in decreased oxygenation of tissues and organs.

Question 17

Which step is NOT part of the DiffQuik staining procedure for blood smears?

Accepted Answer

Heating the slide to 100°C for 10 minutes

Question 18

Which of the following best describes poikilocytosis?

Accepted Answer

Variation in the shape of erythrocytes

Question 19

Why is long-term storage of blood smears generally not necessary in a clinical laboratory setting?

Accepted Answer

Blood smears are used for immediate diagnosis and do not require long-term reference.

Question 20

A blood film is prepared and stored in a dark, dust-free slide box. What is the primary reason for this storage method?

Accepted Answer

To protect the smear from scratches and environmental damage.

Principles and Practice of Clinical Hematology: Microscopic Examination of Peripheral Blood

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